Plant Cell Huazhong Agricultural University
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

First published online January 20, 2009; 10.1105/tpc.108.061309

The Plant Cell 21:54-71 (2009)
© 2009 American Society of Plant Biologists

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow PPT slides of all figures
Right arrow Supplemental Data
Right arrow All Versions of this Article:
21/1/54    most recent
tpc.108.061309v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gao, M.-J.
Right arrow Articles by Rozwadowski, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gao, M.-J.
Right arrow Articles by Rozwadowski, K.
Agricola
Right arrow Articles by Gao, M.-J.
Right arrow Articles by Rozwadowski, K.

Repression of Seed Maturation Genes by a Trihelix Transcriptional Repressor in Arabidopsis Seedlings[W]

Ming-Jun Gao1, Derek J. Lydiate, Xiang Li, Helen Lui, Branimir Gjetvaj, Dwayne D. Hegedus and Kevin Rozwadowski

Agriculture and Agri-Food Canada, Saskatoon Research Centre, Saskatoon, Saskatchewan S7N 0X2, Canada

1 Address correspondence to gaom{at}agr.gc.ca.

The seed maturation program is repressed during germination and seedling development so that embryonic genes are not expressed in vegetative organs. Here, we describe a regulator that represses the expression of embryonic seed maturation genes in vegetative tissues. ASIL1 (for Arabidopsis 6b-interacting protein 1-like 1) was isolated by its interaction with the Arabidopsis thaliana 2S3 promoter. ASIL1 possesses domains conserved in the plant-specific trihelix family of DNA binding proteins and belongs to a subfamily of 6b-interacting protein 1-like factors. The seedlings of asil1 mutants exhibited a global shift in gene expression to a profile resembling late embryogenesis. LEAFY COTYLEDON1 and 2 were markedly derepressed during early germination, as was a large subset of seed maturation genes, such as those encoding seed storage proteins and oleosins, in seedlings of asil1 mutants. Consistent with this, asil1 seedlings accumulated 2S albumin and oil with a fatty acid composition similar to that of seed-derived lipid. Moreover, ASIL1 specifically recognized a GT element that overlaps the G-box and is in close proximity to the RY repeats of the 2S promoters. We suggest that ASIL1 targets GT-box–containing embryonic genes by competing with the binding of transcriptional activators to this promoter region.




This article has been cited by other articles:


Home page
 Plant CellHome page
C. Breuer, A. Kawamura, T. Ichikawa, R. Tominaga-Wada, T. Wada, Y. Kondou, S. Muto, M. Matsui, and K. Sugimoto
The Trihelix Transcription Factor GTL1 Regulates Ploidy-Dependent Cell Growth in the Arabidopsis Trichome
PLANT CELL, August 1, 2009; 21(8): 2307 - 2322.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 2009 by the American Society of Plant Biologists