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First published online January 16, 2009; 10.1105/tpc.108.061812

The Plant Cell 21:90-105 (2009)
© 2009 American Society of Plant Biologists

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ENDOSPERM DEFECTIVE1 Is a Novel Microtubule-Associated Protein Essential for Seed Development in Arabidopsis[W]

Cristina Pignocchi, Gregory E. Minns, Nathalie Nesi1, Rachil Koumproglou, Georgios Kitsios, Christoph Benning2, Clive W. Lloyd, John H. Doonan3 and Matthew J Hills

John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, United Kingdom

3 Address correspondence to john.doonan{at}bbsrc.ac.uk.

Early endosperm development involves a series of rapid nuclear divisions in the absence of cytokinesis; thus, many endosperm mutants reveal genes whose functions are essential for mitosis. This work finds that the endosperm of Arabidopsis thaliana endosperm-defective1 (ede1) mutants never cellularizes, contains a reduced number of enlarged polyploid nuclei, and features an aberrant microtubule cytoskeleton, where the specialized radial microtubule systems and cytokinetic phragmoplasts are absent. Early embryo development is substantially normal, although occasional cytokinesis defects are observed. The EDE1 gene was cloned using a map-based approach and represents the pioneer member of a conserved plant-specific family of genes of previously unknown function. EDE1 is expressed in the endosperm and embryo of developing seeds, and its expression is tightly regulated during cell cycle progression. EDE1 protein accumulates in nuclear caps in premitotic cells, colocalizes along microtubules of the spindle and phragmoplast, and binds microtubules in vitro. We conclude that EDE1 is a novel plant-specific microtubule-associated protein essential for microtubule function during the mitotic and cytokinetic stages that generate the Arabidopsis endosperm and embryo.




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The Metabolic Role of the Legume Endosperm: A Noninvasive Imaging Study
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[Abstract] [Full Text] [PDF]


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