Plant Cell Huazhong Agricultural University
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First published online October 23, 2009; 10.1105/tpc.109.069179

The Plant Cell 21:3170-3184 (2009)
© 2009 American Society of Plant Biologists

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Protein Phosphatases 2C Regulate the Activation of the Snf1-Related Kinase OST1 by Abscisic Acid in Arabidopsis[W]

Florina Vlada, Silvia Rubiob, Americo Rodriguesb, Caroline Sirichandraa, Christophe Belina,1, Nadia Roberta,2, Jeffrey Leunga, Pedro L. Rodriguezb, Christiane Laurièrea,3 and Sylvain Merlota,4

a Centre National de la Recherche Scientifique, Institut des Sciences du Végétal, UPR 2355, 91198 Gif-sur-Yvette Cedex, France
b Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-UPV, ES-46022 Valencia, Spain

3 Address correspondence to christiane.lauriere{at}isv.cnrs-gif.fr.

The plant hormone abscisic acid (ABA) orchestrates plant adaptive responses to a variety of stresses, including drought. This signaling pathway is regulated by reversible protein phosphorylation, and genetic evidence demonstrated that several related protein phosphatases 2C (PP2Cs) are negative regulators of this pathway in Arabidopsis thaliana. Here, we developed a protein phosphatase profiling strategy to define the substrate preferences of the HAB1 PP2C implicated in ABA signaling and used these data to screen for putative substrates. Interestingly, this analysis designated the activation loop of the ABA activated kinase OST1, related to Snf1 and AMPK kinases, as a putative HAB1 substrate. We experimentally demonstrated that HAB1 dephosphorylates and deactivates OST1 in vitro. Furthermore, HAB1 and the related PP2Cs ABI1 and ABI2 interact with OST1 in vivo, and mutations in the corresponding genes strongly affect OST1 activation by ABA. Our results provide evidence that PP2Cs are directly implicated in the ABA-dependent activation of OST1 and further suggest that the activation mechanism of AMPK/Snf1-related kinases through the inhibition of regulating PP2Cs is conserved from plants to human.






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