This Article Full Text Full Text (PDF) PPT slides of all figures Supplemental Data All Versions of this Article: 21/11/3641    most recent tpc.109.070417v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Dissmeyer, N. Articles by Schnittger, A. Search for Related Content PubMed PubMed Citation Articles by Dissmeyer, N. Articles by Schnittger, A. Agricola Articles by Dissmeyer, N. Articles by Schnittger, A.

Control of Cell Proliferation, Organ Growth, and DNA Damage Response Operate Independently of Dephosphorylation of the Arabidopsis Cdk1 Homolog CDKA;1^[C],[W]

^a Unigruppe am Max-Planck-Institut für Züchtungsforschung, Max-Delbrück-Laboratorium, Lehrstuhl für Botanik III, Universität zu Köln, D-50829 Köln, Germany
^b Department of Molecular Mechanisms of Phenotypic Plasticity, Institut de Biologie Moléculaire des Plantes du Centre National de la Recherche Scientifique, Unité Propre de Recherche 2357, Université de Strasbourg, F-67084 Strasbourg Cedex, France
^c Department of Plant Systems Biology, Vlaams Interuniversitair Instituut voor Biotechnologie, B-9052 Gent, Belgium
^d Department of Plant Biotechnology and Genetics, Ghent University, B-9052 Gent, Belgium
^e Oxford Centre for Integrative Systems Biology, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom
^f Research Center for Eco-environmental Sciences, Chinese Academy of Sciences, Beijing 100085, People's Republic of China

³ Address correspondence to arp.schnittger{at}ibmp-ulp.u-strasbg.fr.

Entry into mitosis is universally controlled by cyclin-dependent kinases (CDKs). A key regulatory event in metazoans and fission yeast is CDK activation by the removal of inhibitory phosphate groups in the ATP binding pocket catalyzed by Cdc25 phosphatases. In contrast with other multicellular organisms, we show here that in the flowering plant Arabidopsis thaliana, cell cycle control does not depend on sudden changes in the phosphorylation pattern of the PSTAIRE-containing Cdk1 homolog CDKA;1. Consistently, we found that neither mutants in a previously identified CDC25 candidate gene nor plants in which it is overexpressed display cell cycle defects. Inhibitory phosphorylation of CDKs is also the key event in metazoans to arrest cell cycle progression upon DNA damage. However, we show here that the DNA damage checkpoint in Arabidopsis can also operate independently of the phosphorylation of CDKA;1. These observations reveal a surprising degree of divergence in the circuitry of highly conserved core cell cycle regulators in multicellular organisms. Based on biomathematical simulations, we propose a plant-specific model of how progression through the cell cycle could be wired in Arabidopsis.

This article has been cited by other articles:

				N. D. Spadafora, J. H. Doonan, R. J. Herbert, M. B. Bitonti, E. Wallace, H. J. Rogers, and D. Francis Arabidopsis T-DNA insertional lines for CDC25 are hypersensitive to hydroxyurea but not to zeocin or salt stress Ann. Bot., July 20, 2010; (2010) mcq142v1. [Abstract] [Full Text] [PDF]

				R. Kasili, J. D. Walker, L. A. Simmons, J. Zhou, L. De Veylder, and J. C. Larkin SIAMESE Cooperates With the CDH1-like Protein CCS52A1 to Establish Endoreplication in Arabidopsis thaliana Trichomes Genetics, May 1, 2010; 185(1): 257 - 268. [Abstract] [Full Text] [PDF]