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Genetic Regulation of Meiotic Cross-Overs between Related Genomes in Brassica napus Haploids and Hybrids^[C],[W]

^a Institut National de la Recherche Agronomique, Unité Mixte de Recherche 118, Amélioration des Plantes et Biotechnologies Végétales, F-35653 Le Rheu, France
^b Institut National de la Recherche Agronomique–Unité de Mathématique et Informatique Appliquées, Unité de Recherche 341, F-78350 Jouy-en-Josas, France
^c Institut National de la Recherche Agronomique–Institut Jean Pierre Bourgin, Station de Génétique et Amélioration des Plantes, F-78026 Versailles Cedex, France

³ Address correspondence to ejenczewski{at}versailles.inra.fr.

Although the genetic regulation of recombination in allopolyploid species plays a pivotal role in evolution and plant breeding, it has received little recent attention, except in wheat (Triticum aestivum). PrBn is the main locus that determines the number of nonhomologous associations during meiosis of microspore cultured Brassica napus haploids (AC; 19 chromosomes). In this study, we examined the role played by PrBn in recombination. We generated two haploid x euploid populations using two B. napus haploids with differing PrBn (and interacting genes) activity. We analyzed molecular marker transmission in these two populations to compare genetic changes, which have arisen during meiosis. We found that cross-over number in these two genotypes was significantly different but that cross-overs between nonhomologous chromosomes showed roughly the same distribution pattern. We then examined genetic recombination along a pair of A chromosomes during meiosis of B. rapa x B. napus AAC and AACC hybrids that were produced with the same two B. napus genotypes. We observed significant genotypic variation in cross-over rates between the two AAC hybrids but no difference between the two AACC hybrids. Overall, our results show that PrBn changes the rate of recombination between nonhomologous chromosomes during meiosis of B. napus haploids and also affects homologous recombination with an effect that depends on plant karyotype.

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