This Article Full Text Full Text (PDF) Supplemental Data Author Profile All Versions of this Article: 21/5/1541    most recent tpc.108.064279v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in Plant Cell Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Raffaele, S. Articles by Mongrand, S. Search for Related Content PubMed PubMed Citation Articles by Raffaele, S. Articles by Mongrand, S. Agricola Articles by Raffaele, S. Articles by Mongrand, S.

Remorin, a Solanaceae Protein Resident in Membrane Rafts and Plasmodesmata, Impairs Potato virus X Movement^[W]

^a Laboratoire de Biogenèse Membranaire, Unité Mixte de Recherche 5200, Centre National de la Recherche Scientifique-University of Bordeaux, Bordeaux 33076, France
^b Institute of Plant Sciences, University of Bern, 3013 Bern, Switzerland
^c Department of Plant Molecular Biology, University of Lausanne, CH-1015 Lausanne-Dorigny, Switzerland
^d Unité Mixte de Recherche, Génomique, Diversité et Pouvoir Pathogène 1090, Institut National de la Recherche Agronomique, University of Bordeaux 2, Interaction Plantes Virus, 33883 Villenave d'Ornon, France
^e Laboratoire Bordelais de Recherche en Informatique, Unité Mixte de Recherche 5800, Centre National de la Recherche Scientifique-University of Bordeaux, Bordeaux 33076, France
^f Unité Mixte de Recherche, Plante-Microbe-Environnement 1088, Institut National de la Recherche Agronomique-5184, Centre National de la Recherche Scientifique-Université de Bourgogne, 21065 Dijon, France
^g Plateau Technique Imagerie/Cytologie, Institut National de la Recherche Agronomique, Institut Fédératif de Recherche 103, 33883 Villenave d'Ornon, France
^h Laboratoire Dynamique de la Compartimentation Cellulaire, Institut des Sciences du Végétal, Centre National de la Recherche Scientifique, Unité Propre de Recherche 2355, 91198, Gif sur Yvette, France
ⁱ Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594/441, 31326 Castanet-Tolosan, France
^j John Innes Centre, Colney, Norwich NR4 7UH, United Kingdom

⁶ Address correspondence to sebastien.mongrand{at}biomemb.u-bordeaux2.fr.

Remorins (REMs) are proteins of unknown function specific to vascular plants. We have used imaging and biochemical approaches and in situ labeling to demonstrate that REM clusters at plasmodesmata and in 70-nm membrane domains, similar to lipid rafts, in the cytosolic leaflet of the plasma membrane. From a manipulation of REM levels in transgenic tomato (Solanum lycopersicum) plants, we show that Potato virus X (PVX) movement is inversely related to REM accumulation. We show that REM can interact physically with the movement protein TRIPLE GENE BLOCK PROTEIN1 from PVX. Based on the localization of REM and its impact on virus macromolecular trafficking, we discuss the potential for lipid rafts to act as functional components in plasmodesmata and the plasma membrane.

Related articles in Plant Cell:

Membrane Rafts and Virus Movement in Plant Cells

Nancy A. Eckardt
Plant Cell 2009 21: 1326. [Full Text]  

This article has been cited by other articles:

				B. Lefebvre, T. Timmers, M. Mbengue, S. Moreau, C. Herve, K. Toth, J. Bittencourt-Silvestre, D. Klaus, L. Deslandes, L. Godiard, et al. A remorin protein interacts with symbiotic receptors and regulates bacterial infection PNAS, February 2, 2010; 107(5): 2343 - 2348. [Abstract] [Full Text] [PDF]

				N. A. Eckardt Membrane Rafts and Virus Movement in Plant Cells PLANT CELL, May 1, 2009; 21(5): 1326 - 1326. [Full Text] [PDF]