This Article Full Text Full Text (PDF) PPT slides of all figures Supplemental Data All Versions of this Article: 21/7/1940    most recent tpc.109.066373v1 Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (3) Citing Articles via Google Scholar Google Scholar Articles by Brownfield, L. Articles by Twell, D. Search for Related Content PubMed PubMed Citation Articles by Brownfield, L. Articles by Twell, D. Pubmed/NCBI databases Gene GEO Profiles Protein UniGene Agricola Articles by Brownfield, L. Articles by Twell, D.

Arabidopsis DUO POLLEN3 Is a Key Regulator of Male Germline Development and Embryogenesis^[C],[W]

^a Department of Biology, University of Leicester, Leicester LE1 7RH, United Kingdom
^b Institute for Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JH, United Kingdom

¹ Address correspondence to twe{at}le.ac.uk.

Male germline development in angiosperms produces the pair of sperm cells required for double fertilization. A key regulator of this process in Arabidopsis thaliana is the male germline-specific transcription factor DUO POLLEN1 (DUO1) that coordinates germ cell division and gamete specification. Here, we uncover the role of DUO3, a nuclear protein that has a distinct, but overlapping role with DUO1 in male germline development. DUO3 is a conserved protein in land plants and is related to GON-4, a cell lineage regulator of gonadogenesis in Caenorhabditis elegans. Mutant duo3-1 germ cells either fail to divide or show a delay in division, and we show that, unlike DUO1, DUO3 promotes entry into mitosis independent of the G2/M regulator CYCB1;1. We also show that DUO3 is required for the expression of a subset of germline genes under DUO1 control and that like DUO1, DUO3 is essential for sperm cell specification and fertilization. Furthermore, we demonstrate an essential sporophytic role for DUO3 in cell division and embryo patterning. Our findings demonstrate essential developmental roles for DUO3 in cell cycle progression and cell specification in both gametophytic and sporophytic tissues.

This article has been cited by other articles:

				G. Le Trionnaire and D. Twell Small RNAs in angiosperm gametophytes: from epigenetics to gamete development Genes & Dev., June 1, 2010; 24(11): 1081 - 1085. [Abstract] [Full Text] [PDF]

				A. C. Frank and M. A. Johnson Expressing the Diphtheria Toxin A Subunit from the HAP2(GCS1) Promoter Blocks Sperm Maturation and Produces Single Sperm-Like Cells Capable of Fertilization Plant Physiology, November 1, 2009; 151(3): 1390 - 1400. [Abstract] [Full Text] [PDF]