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THE PLANT CELL, Vol 3, Issue 11 1155-1165, Copyright © 1991 by American Society of Plant Biologists
Analysis of Rice Act1 5[prime] Region Activity in Transgenic Rice Plants
W. Zhang, D. McElroy and R. Wu
Field of Botany, Cornell University, Ithaca, New York 14853
The 5[prime] region of the rice actin 1 gene (Act1) has been developed as
an efficient regulator of foreign gene expression in transgenic rice
plants. To determine the pattern and level of rice Act1 5[prime] region
activity, transgenic rice plants containing the Act1 5[prime] region fused
to a bacterial [beta]-glucuronidase (Gus) coding sequence were generated.
Two independent clonal lines of transgenic rice plants were analyzed in
detail. Quantitative analysis showed that tissue from these transgenic rice
plants have a level of GUS protein that represents as much as 3% of total
soluble protein. We were able to demonstrate that Act1-Gus gene expression
is constitutive throughout the sporophytic and gametophytic tissues of
these transgenic rice plants. Plants from one transgenic line were analyzed
for the segregation of GUS activity in pollen by in situ histochemical
staining, and the inheritance and stability of Act1-Gus expression were
assayed in subsequently derived progeny plants.
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