|
THE PLANT CELL, Vol 3, Issue 11 1187-1193, Copyright © 1991 by American Society of Plant Biologists
Control of Ethylene Synthesis by Expression of a Bacterial Enzyme in Transgenic Tomato Plants
H. J. Klee, M. B. Hayford, K. A. Kretzmer, G. F. Barry and G. M. Kishore
Monsanto Company, 700 Chesterfield Village Parkway, Chesterfield, Missouri 63198
Synthesis of the phytohormone ethylene is believed to be essential for many
plant developmental processes. The control of ripening in climacteric
fruits and vegetables is among the best characterized of these processes.
One approach to reduce ethylene synthesis in plants is metabolism of its
immediate precursor, 1-aminocyclopropane-1-carboxylic acid (ACC). Soil
bacteria containing an enzyme, ACC deaminase, were identified by their
ability to grow on ACC as a sole nitrogen source. The gene encoding ACC
deaminase was cloned and introduced into tomato plants. Reduction in
ethylene synthesis in transgenic plants did not cause any apparent
vegetative phenotypic abnormalities. However, fruits from these plants
exhibited significant delays in ripening, and the mature fruits remained
firm for at least 6 weeks longer than the nontransgenic control fruit.
These results indicated that ACC deaminase is useful for examining the role
of ethylene in many developmental and stress-related processes in plants as
well as for extending the shelf life of fruits and vegetables whose
ripening is mediated by ethylene.
This article has been cited by other articles:
|
|
|
|
 
V. Balaji, M. Mayrose, O. Sherf, J. Jacob-Hirsch, R. Eichenlaub, N. Iraki, S. Manulis-Sasson, G. Rechavi, I. Barash, and G. Sessa
Tomato Transcriptional Changes in Response to Clavibacter michiganensis subsp. michiganensis Reveal a Role for Ethylene in Disease Development
Plant Physiology,
April 1, 2008;
146(4):
1797 - 1809.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Joardar, M. Lindeberg, R. W. Jackson, J. Selengut, R. Dodson, L. M. Brinkac, S. C. Daugherty, R. DeBoy, A. S. Durkin, M. G. Giglio, et al.
Whole-Genome Sequence Analysis of Pseudomonas syringae pv. phaseolicola 1448A Reveals Divergence among Pathovars in Genes Involved in Virulence and Transposition
J. Bacteriol.,
September 15, 2005;
187(18):
6488 - 6498.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Block, E. Schmelz, P. J. O'Donnell, J. B. Jones, and H. J. Klee
Systemic Acquired Tolerance to Virulent Bacterial Pathogens in Tomato
Plant Physiology,
July 1, 2005;
138(3):
1481 - 1490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Tsuchisaka and A. Theologis
Heterodimeric interactions among the 1-amino-cyclopropane-1-carboxylate synthase polypeptides encoded by the Arabidopsis gene family
PNAS,
February 24, 2004;
101(8):
2275 - 2280.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ose, A. Fujino, M. Yao, N. Watanabe, M. Honma, and I. Tanaka
Reaction Intermediate Structures of 1-Aminocyclopropane-1-carboxylate Deaminase: INSIGHT INTO PLP-DEPENDENT CYCLOPROPANE RING-OPENING REACTION
J. Biol. Chem.,
October 17, 2003;
278(42):
41069 - 41076.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Dammann, A. Ichida, B. Hong, S. M. Romanowsky, E. M. Hrabak, A. C. Harmon, B. G. Pickard, and J. F. Harper
Subcellular Targeting of Nine Calcium-Dependent Protein Kinase Isoforms from Arabidopsis
Plant Physiology,
August 1, 2003;
132(4):
1840 - 1848.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Moore, J. Vrebalov, P. Payton, and J. Giovannoni
Use of genomics tools to isolate key ripening genes and analyse fruit maturation in tomato
J. Exp. Bot.,
October 1, 2002;
53(377):
2023 - 2030.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Trott, S. Burger, C. Calaminus, and A. Stolz
Cloning and Heterologous Expression of an Enantioselective Amidase from Rhodococcus erythropolis Strain MP50
Appl. Envir. Microbiol.,
July 1, 2002;
68(7):
3279 - 3286.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Diaz, A. ten Have, and J. A.L. van Kan
The Role of Ethylene and Wound Signaling in Resistance of Tomato to Botrytis cinerea
Plant Physiology,
July 1, 2002;
129(3):
1341 - 1351.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Tian and Z. J. Chen
Blocking histone deacetylation in Arabidopsis induces pleiotropic effects on plant gene regulation and development
PNAS,
December 22, 2000;
(2000)
11347998.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Hoffman, J. S. Schmidt, X. Zheng, and A. F. Bent
Isolation of Ethylene-Insensitive Soybean Mutants That Are Altered in Pathogen Susceptibility and Gene-for-Gene Disease Resistance
Plant Physiology,
March 1, 1999;
119(3):
935 - 950.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. S. Tarun, J. S. Lee, and A. Theologis
Random mutagenesis of 1-aminocyclopropane-1-carboxylate synthase: A key enzyme in ethylene biosynthesis
PNAS,
August 18, 1998;
95(17):
9796 - 9801.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. T. Lund, R. E. Stall, and H. J. Klee
Ethylene Regulates the Susceptible Response to Pathogen Infection in Tomato
PLANT CELL,
March 1, 1998;
10(3):
371 - 382.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. B. Dotson, C. E. Smith, C. S. Ling, G. F. Barry, and G. M. Kishore
Identification, Characterization, and Cloning of a Phosphonate Monoester Hydrolase from Burkholderia caryophilli PG2982
J. Biol. Chem.,
October 18, 1996;
271(42):
25754 - 25761.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Q. Wilkinson, M. B. Lanahan, H.-C. Yen, J. J. Giovannoni, and H. J. Klee
An Ethylene-Inducible Component of Signal Transduction Encoded by Never-ripe
Science,
December 15, 1995;
270(5243):
1807 - 1809.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. Yao, T. Ose, H. Sugimoto, A. Horiuchi, A. Nakagawa, S. Wakatsuki, D. Yokoi, T. Murakami, M. Honma, and I. Tanaka
Crystal Structure of 1-Aminocyclopropane-1-carboxylate Deaminase from Hansenula saturnus
J. Biol. Chem.,
October 27, 2000;
275(44):
34557 - 34565.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Tian and Z. J. Chen
Blocking histone deacetylation in Arabidopsis induces pleiotropic effects on plant gene regulation and development
PNAS,
January 2, 2001;
98(1):
200 - 205.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
