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THE PLANT CELL, Vol 3, Issue 11 1239-1250, Copyright © 1991 by American Society of Plant Biologists


RESEARCH ARTICLES

A Scaffold-Associated DNA Region Is Located Downstream of the Pea Plastocyanin Gene

R. E. Slatter, P. Dupree and J. C. Gray
Botany School, University of Cambridge, Downing Street, Cambridge CB2 3EA, United Kingdom

Chromosomal scaffold-associated DNA has been isolated from pea leaf nuclei treated with lithium diiodosalicylate to remove histones and then digested with restriction enzymes to remove the DNA in chromosomal loops. A scaffold-associated region (SAR) of DNA has been identified 8 to 9 kb downstream of the single-copy pea plastocyanin gene in proximity to a repetitive sequence present in 300 copies in the pea haploid genome. Isolated restriction fragments from within the SAR can bind to scaffold preparations in a binding assay in vitro. The nucleotide sequence of the SAR indicates a 540-bp 77% A+T-rich region containing many sequence elements in common with SARs from other organisms. Sequences with homology to topoisomerase II binding sites, A-box and T-box sequences, and replication origins are present within this AT-rich region.


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Copyright © 1991 by the American Society of Plant Biologists