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THE PLANT CELL, Vol 3, Issue 2 175-189, Copyright © 1991 by American Society of Plant Biologists
Chloroplast RNA Stability in Chlamydomonas: Rapid Degradation of psbB and psbC Transcripts in Two Nuclear Mutants
L. E. Sieburth, S. Berry-Lowe and G. W. Schmidt
Department of Botany, University of Georgia, Athens, Georgia 30602
Toward understanding regulation of chloroplast transcript abundance, we
have isolated and analyzed nuclear mutant strains of Chlamydomonas
reinhardtii that lack chloroplast-encoded mRNAs for photosystem II
proteins. Mutant 6.2z5 accumulates no transcripts of the psbC locus for the
43-kilodalton chlorophyll-binding protein. In mutant GE2.10, transcripts of
psbB, encoding the 47-kilodalton chlorophyll-binding protein, cannot be
detected [Jensen, K.H., Herrin, D.L., Plumley, F.G., and Schmidt, G.W.
(1986). J. Cell Biol. 103, 1315-1325]. Also, GE2.10 does not accumulate
several low molecular weight transcripts from a region of the chloroplast
genome proximal to psbB. The levels of mRNAs from other chloroplast genes
are not affected in either mutant. Chloroplast transcription was analyzed
in permeabilized cells and by in vivo pulse labeling. Although 5[prime]
ribonuclease was found as an artifactual activity of permeabilized cells,
the results from both assays demonstrated that wild-type levels of psbC
transcription occur in mutant 6.2z5 and that chloroplasts of GE2.10
transcribe psbB and adjacent genes. Thus, it appears that the nuclear genes
that are mutated in 6.2z5 and GE2.10 encode products that, respectively,
confer stability to transcripts from the psbC and the psbB regions of the
chloroplast genome.
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