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THE PLANT CELL, Vol 3, Issue 3 271-283, Copyright © 1991 by American Society of Plant Biologists
Action of the Style Product of the Self-Incompatibility Gene of Nicotiana alata (S-RNase) on in Vitro-Grown Pollen Tubes
J. E. Gray, B. A. McClure, I. Bonig, M. A. Anderson and A. E. Clarke
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria 3052, Australia
The products of the S-locus expressed in female tissues of Nicotiana alata
are ribonucleases (S-RNases). The arrest of growth of incompatible pollen
tubes in styles may result from entry of the S-RNase into the pollen tube
and degradation of pollen tube RNA. We investigated the action of isolated
S-RNases on pollen tubes grown in vitro and found that S-RNase is taken up
by the pollen without substantial alteration. The S-RNases inhibit
incorporation of exogenously added radioactive amino acids into protein by
the germinated pollen. The S-RNases also inhibit in vitro translation of
pollen tube RNA in a wheat germ cell-free extract. We found no evidence for
a specific mRNA substrate for the S-RNases, which implies that if RNase
activity is involved in the control of self-incompatibility, allelic
specificity is more likely to depend on the selective uptake of S-RNases
into pollen tubes or their selective activation or inactivation by pollen
factors, rather than cleavage of a specific substrate. Heat treating
S2-RNase largely destroys its RNase activity but increases its inhibitory
effect on in vitro pollen tube growth. This effect is not due to an
increased uptake of S2-RNase by the pollen but is associated with a greatly
enhanced accumulation of S2-RNase on the outer surface of the pollen
grains.
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