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THE PLANT CELL, Vol 3, Issue 4 359-370, Copyright © 1991 by American Society of Plant Biologists
Molecular Cloning and Characterization of Genes Expressed in Shoot Apical Meristems
J. I. Medford, J. S. Elmer and H. J. Klee
Plant Molecular Biology Group, Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, Missouri 63198
The above-ground portion of a plant develops from the shoot apical
meristem. An abundant source of apical meristems was obtained from
cauliflower heads. Meristematic cDNAs were identified by differential
screening and used to isolate corresponding Arabidopsis thaliana genes.
Transcriptional promoters from Arabidopsis clones were fused to the
[beta]-glucuronidase (GUS) reporter gene and introduced into plants, and
GUS expression was used to analyze temporal and spatial regulation of the
promoters. One promoter (meri-5) directed GUS expression in the
meristematic dome and not the surrounding leaf primordia. The meri-5
promoter also directed GUS expression at branching points in the shoot and
root. A second meristematic gene was found to be a histone (H3) gene. The
H3 promoter was isolated and fused to GUS. Expression of the H3-GUS fusion
in transgenic tobacco showed preferential expression in the peripheral zone
and a lack of noticeable staining in the central zone.
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