Plant Cell
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (96)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yamamoto, Y. T.
Right arrow Articles by Conkling, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yamamoto, Y. T.
Right arrow Articles by Conkling, M. A.
Agricola
Right arrow Articles by Yamamoto, Y. T.
Right arrow Articles by Conkling, M. A.

THE PLANT CELL, Vol 3, Issue 4 371-382, Copyright © 1991 by American Society of Plant Biologists

RESEARCH ARTICLES

Characterization of cis-Acting Sequences Regulating Root-Specific Gene Expression in Tobacco

Y. T. Yamamoto, C. G. Taylor, G. N. Acedo, C. L. Cheng and M. A. Conkling
Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695

The expression of the tobacco root-specific gene TobRB7 was characterized. Gel blot hybridizations to RNA isolated from various tobacco tissues demonstrated that steady-state TobRB7 mRNA is not detected in expanded leaf, stem, or shoot apex tissue. To determine the spatial pattern of expression, in situ hybridization to root sections revealed that TobRB7 expression is localized to root meristem and immature central cylinder regions. The 5[prime] flanking region of the gene was studied with respect to its ability to direct root-specific expression. Deletions of 5[prime] flanking sequence were fused to the [beta]-glucuronidase (GUS) reporter gene and transformed into tobacco. Our data demonstrated that sequences 636 base pairs from the site of transcription initiation are sufficient to direct the root-specific GUS expression in transgenic tobacco, whereas sequences 299 base pairs from the site of transcription initiation fail to direct root-specific expression. A negative regulatory element was apparent between 813 base pairs and 636 base pairs 5[prime] of the transcription initiation site. Histochemical localization of GUS activity in transgenic plants was consistent with in situ hybridization results: GUS activity was localized to the root meristem and central cylinder regions. GUS activity appeared 2 days post-germination in the primary root meristem. In lateral roots, GUS activity was detected from the time of initiation.


This article has been cited by other articles:


Home page
 J Exp BotHome page
S. P. Vaughan, D. J. James, K. Lindsey, and A. J. Massiah
Characterization of FaRB7, a near root-specific gene from strawberry (Fragariaxananassa Duch.) and promoter activity analysis in homologous and heterologous hosts
J. Exp. Bot., November 1, 2006; 57(14): 3901 - 3910.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Brand, M. Horler, E. Nuesch, S. Vassalli, P. Barrell, W. Yang, R. A. Jefferson, U. Grossniklaus, and M. D. Curtis
A Versatile and Reliable Two-Component System for Tissue-Specific Gene Induction in Arabidopsis
Plant Physiology, August 1, 2006; 141(4): 1194 - 1204.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
H. An, C. Roussot, P. Suarez-Lopez, L. Corbesier, C. Vincent, M. Pineiro, S. Hepworth, A. Mouradov, S. Justin, C. Turnbull, et al.
CONSTANS acts in the phloem to regulate a systemic signal that induces photoperiodic flowering of Arabidopsis
Development, August 1, 2004; 131(15): 3615 - 3626.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
H. JAVOT and C. MAUREL
The Role of Aquaporins in Root Water Uptake
Ann. Bot., September 1, 2002; 90(3): 301 - 313.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
L. Jiang, T. E. Phillips, S. W. Rogers, and J. C. Rogers
Biogenesis of the Protein Storage Vacuole Crystalloid
J. Cell Biol., August 21, 2000; 150(4): 755 - 770.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H.-H. Kirch, R. Vera-Estrella, D. Golldack, F. Quigley, C. B. Michalowski, B. J. Barkla, and H. J. Bohnert
Expression of Water Channel Proteins in Mesembryanthemum crystallinum
Plant Physiology, May 1, 2000; 123(1): 111 - 124.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
F. Chaumont, F. Barrieu, E. M. Herman, and M. J. Chrispeels
Characterization of a Maize Tonoplast Aquaporin Expressed in Zones of Cell Division and Elongation
Plant Physiology, August 1, 1998; 117(4): 1143 - 1152.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
F. Barrieu, F. Chaumont, and M. J. Chrispeels
High Expression of the Tonoplast Aquaporin ZmTIP1 in Epidermal and Conducting Tissues of Maize
Plant Physiology, August 1, 1998; 117(4): 1153 - 1163.
[Abstract] [Full Text]

Home page
 ScienceHome page
C. H. Opperman, C. G. Taylor, and M. A. Conkling
Root-Knot Nematode--Directed Expression of a Plant Root--Specific Gene
Science, January 14, 1994; 263(5144): 221 - 223.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1991 by the American Society of Plant Biologists