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THE PLANT CELL, Vol 3, Issue 4 419-430, Copyright © 1991 by American Society of Plant Biologists
Tissue-Specific and Organ-Specific Expression of Soybean Auxin-Responsive Transcripts GH3 and SAURs
M. A. Gee, G. Hagen and T. J. Guilfoyle
Department of Biology, University of Missouri-Columbia, Columbia, Missouri 65211
We used in situ hybridization to localize two classes of auxin-regulated
transcripts, GH3 and SAURs, within organs and tissues of soybean seedlings
and flowers. GH3 transcripts occurred in the inner cortex and protoxylem
ridges of roots and were expressed transiently during flower and pod
development. SAUR transcripts were expressed in the epidermis, cortex, and
starch sheath of epicotyls and immature hypocotyls. SAUR transcripts became
more abundant on the bottom side of hypocotyls that were undergoing
gravitropic curvature. SAURs were also expressed in developing xylem
elements of the hypocotyl hook. When soybean organ sections were treated
with 50 micromolar 2,4-dichlorophenoxyacetic acid (2,4-D), GH3 transcripts
became more abundant in the vascular regions of all organs analyzed. High
levels of GH3 transcripts were also found in developing palisade mesophyll
cells of leaves, cotyledons, and flowers treated with 2,4-D. SAUR
transcripts became more abundant in the epidermis, cortex, starch sheath,
and pith of epicotyls and hypocotyls after 2,4-D treatment. Our results
showed that a variety of tissues and cell types express auxin-responsive
transcripts and that different tissues respond rapidly to exogenous auxin
by expressing different hormone-responsive genes.
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