THE PLANT CELL, Vol 3, Issue 5 483-496, Copyright © 1991 by American Society of Plant Biologists

RESEARCH ARTICLES

Characterization of an Immunoglobulin Binding Protein Homolog in the Maize floury-2 Endosperm Mutant

EBP. Fontes, B. B. Shank, R. L. Wrobel, S. P. Moose, G. R. OBrian, E. T. Wurtzel and R. S. Boston
Department of Botany, Box 7612, North Carolina State University, Raleigh, North Carolina 27695-7612

The maize b-70 protein is an endoplasmic reticulum protein overproduced in the floury-2 (fl2) endosperm mutant. The increase in b-70 levels in fl2 plants occurs during seed maturation and is endosperm specific. We have used amino acid sequence homology to identify b-70 as a homolog of mammalian immunoglobulin binding protein (BiP). Purified b-70 fractions contain two 75-kilodalton polypeptides with pl values of 5.3 and 5.4. Both 75-kilodalton polypeptides share several properties with BiP, including the ability to bind ATP and localization within the lumen of the endoplasmic reticulum. In addition, both b-70 polypeptides can be induced in maize cell cultures with tunicamycin treatment. Like BiP, the pl 5.3 form of b-70 is post-translationally modified by phosphorylation and ADP-ribosylation. However, modification of the pl 5.4 species was not detected in vitro or in vivo. Although the b-70 gene is unlinked to fl2, b-70 overproduction is positively correlated with the fl2 gene and is regulated at the mRNA level. In contrast, the fl2 allele negatively affects the accumulation of the major endosperm storage proteins. The physical similarity of b-70 to BiP and its association with abnormal protein accumulation in fl2 endoplasmic reticulum may reflect a biological function to mediate protein folding and assembly in maize endosperm.

This article has been cited by other articles:

				Y. Iwata, N. V. Fedoroff, and N. Koizumi Arabidopsis bZIP60 Is a Proteolysis-Activated Transcription Factor Involved in the Endoplasmic Reticulum Stress Response PLANT CELL, November 1, 2008; 20(11): 3107 - 3121. [Abstract] [Full Text] [PDF]

				F. Reyes, L. Marchant, L. Norambuena, R. Nilo, H. Silva, and A. Orellana AtUTr1, a UDP-glucose/UDP-galactose Transporter from Arabidopsis thaliana, Is Located in the Endoplasmic Reticulum and Up-regulated by the Unfolded Protein Response J. Biol. Chem., April 7, 2006; 281(14): 9145 - 9151. [Abstract] [Full Text] [PDF]

				P. Pimpl, J. P. Taylor, C. Snowden, S. Hillmer, D. G. Robinson, and J. Denecke Golgi-Mediated Vacuolar Sorting of the Endoplasmic Reticulum Chaperone BiP May Play an Active Role in Quality Control within the Secretory Pathway PLANT CELL, January 1, 2006; 18(1): 198 - 211. [Abstract] [Full Text] [PDF]

				A. Heese, A. A. Ludwig, and J. D.G. Jones Rapid Phosphorylation of a Syntaxin during the Avr9/Cf-9-Race-Specific Signaling Pathway Plant Physiology, August 1, 2005; 138(4): 2406 - 2416. [Abstract] [Full Text] [PDF]

				H.-J. Ju, T. D. Samuels, Y.-S. Wang, E. Blancaflor, M. Payton, R. Mitra, K. Krishnamurthy, R. S. Nelson, and J. Verchot-Lubicz The Potato Virus X TGBp2 Movement Protein Associates with Endoplasmic Reticulum-Derived Vesicles during Virus Infection Plant Physiology, August 1, 2005; 138(4): 1877 - 1895. [Abstract] [Full Text] [PDF]

				M. E. Kirst, D. J. Meyer, B. C. Gibbon, R. Jung, and R. S. Boston Identification and Characterization of Endoplasmic Reticulum-Associated Degradation Proteins Differentially Affected by Endoplasmic Reticulum Stress Plant Physiology, May 1, 2005; 138(1): 218 - 231. [Abstract] [Full Text] [PDF]

				H. Washida, A. Sugino, J. Messing, A. Esen, and T. W. Okita Asymmetric Localization of Seed Storage Protein RNAs to Distinct Subdomains of the Endoplasmic Reticulum in Developing Maize Endosperm Cells Plant Cell Physiol., December 15, 2004; 45(12): 1830 - 1837. [Abstract] [Full Text] [PDF]

				P. D. Matthews, R. Luo, and E. T. Wurtzel Maize phytoene desaturase and {zeta}-carotene desaturase catalyse a poly-Z desaturation pathway: implications for genetic engineering of carotenoid content among cereal crops J. Exp. Bot., October 1, 2003; 54(391): 2215 - 2230. [Abstract] [Full Text] [PDF]

				S. L. Stone, E. M. Anderson, R. T. Mullen, and D. R. Goring ARC1 Is an E3 Ubiquitin Ligase and Promotes the Ubiquitination of Proteins during the Rejection of Self-Incompatible Brassica Pollen PLANT CELL, April 1, 2003; 15(4): 885 - 898. [Abstract] [Full Text] [PDF]

				I. M. Martinez and M. J. Chrispeels Genomic Analysis of the Unfolded Protein Response in Arabidopsis Shows Its Connection to Important Cellular Processes PLANT CELL, February 1, 2003; 15(2): 561 - 576. [Abstract] [Full Text] [PDF]

				B. G. Hunter, M. K. Beatty, G. W. Singletary, B. R. Hamaker, B. P. Dilkes, B. A. Larkins, and R. Jung Maize Opaque Endosperm Mutations Create Extensive Changes in Patterns of Gene Expression PLANT CELL, October 1, 2002; 14(10): 2591 - 2612. [Abstract] [Full Text] [PDF]

				N. Koizumi, I. M. Martinez, Y. Kimata, K. Kohno, H. Sano, and M. J. Chrispeels Molecular Characterization of Two Arabidopsis Ire1 Homologs, Endoplasmic Reticulum-Located Transmembrane Protein Kinases Plant Physiology, November 1, 2001; 127(3): 949 - 962. [Abstract] [Full Text] [PDF]

				I. Heilmann, I. Y. Perera, W. Gross, and W. F. Boss Plasma Membrane Phosphatidylinositol 4,5-Bisphosphate Levels Decrease with Time in Culture Plant Physiology, August 1, 2001; 126(4): 1507 - 1518. [Abstract] [Full Text] [PDF]

				F. C. Alvim, S. M.B. Carolino, J. C.M. Cascardo, C. C. Nunes, C. A. Martinez, W. C. Otoni, and E. P.B. Fontes Enhanced Accumulation of BiP in Transgenic Plants Confers Tolerance to Water Stress Plant Physiology, July 1, 2001; 126(3): 1042 - 1054. [Abstract] [Full Text] [PDF]

				K. J. Shank, P. Su, I. Brglez, W. F. Boss, R. E. Dewey, and R. S. Boston Induction of Lipid Metabolic Enzymes during the Endoplasmic Reticulum Stress Response in Plants Plant Physiology, May 1, 2001; 126(1): 267 - 277. [Abstract] [Full Text]

				M. Benghezal, G. O. Wasteneys, and D. A. Jones The C-Terminal Dilysine Motif Confers Endoplasmic Reticulum Localization to Type I Membrane Proteins in Plants PLANT CELL, July 1, 2000; 12(7): 1179 - 1202. [Abstract] [Full Text]

				J. C. M. Cascardo, R. S. Almeida, R. A. A. Buzeli, S. M. B. Carolino, W. C. Otoni, and E. P. B. Fontes The Phosphorylation State and Expression of Soybean BiP Isoforms Are Differentially Regulated following Abiotic Stresses J. Biol. Chem., May 5, 2000; 275(19): 14494 - 14500. [Abstract] [Full Text] [PDF]

				S. Pagny, M. Cabanes-Macheteau, J. W. Gillikin, N. Leborgne-Castel, P. Lerouge, R. S. Boston, L. Faye, and V. Gomord Protein Recycling from the Golgi Apparatus to the Endoplasmic Reticulum in Plants and Its Minor Contribution to Calreticulin Retention PLANT CELL, May 1, 2000; 12(5): 739 - 756. [Abstract] [Full Text]

				R. T. Mullen, C. S. Lisenbee, J. A. Miernyk, and R. N. Trelease Peroxisomal Membrane Ascorbate Peroxidase Is Sorted to a Membranous Network That Resembles a Subdomain of the Endoplasmic Reticulum PLANT CELL, November 1, 1999; 11(11): 2167 - 2186. [Abstract] [Full Text]

				J. A. Miernyk Protein Folding in the Plant Cell Plant Physiology, November 1, 1999; 121(3): 695 - 703. [Full Text]

				N. Leborgne-Castel, E. P. W. M. Jelitto-Van Dooren, A. J. Crofts, and J. Denecke Overexpression of BiP in Tobacco Alleviates Endoplasmic Reticulum Stress PLANT CELL, March 1, 1999; 11(3): 459 - 470. [Abstract] [Full Text] [PDF]

				M. Heinlein, H. S. Padgett, J. S. Gens, B. G. Pickard, S. J. Casper, B. L. Epel, and R. N. Beachy Changing Patterns of Localization of the Tobacco Mosaic Virus Movement Protein and Replicase to the Endoplasmic Reticulum and Microtubules during Infection PLANT CELL, July 1, 1998; 10(7): 1107 - 1120. [Abstract] [Full Text]

				A. J. Crofts, N. Leborgne-Castel, M. Pesca, A. Vitale, and J. Denecke BiP and Calreticulin Form an Abundant Complex That Is Independent of Endoplasmic Reticulum Stress PLANT CELL, May 1, 1998; 10(5): 813 - 824. [Abstract] [Full Text] [PDF]

				Y. Yu, H. He Mu, C. Mu-Forster, and B. P. Wasserman Polypeptides of the Maize Amyloplast Stroma . Stromal Localization of Starch-Biosynthetic Enzymes and Identification of an 81-Kilodalton Amyloplast Stromal Heat-Shock Cognate Plant Physiology, April 1, 1998; 116(4): 1451 - 1460. [Abstract] [Full Text]

				C. E. Coleman, A. M. Clore, J. P. Ranch, R. Higgins, M. A. Lopes, and B. A. Larkins Expression of a mutant alpha -zein creates the floury2 phenotype in transgenic maize PNAS, June 24, 1997; 94(13): 7094 - 7097. [Abstract] [Full Text] [PDF]

				S P Moose and P H Sisco Glossy15, an APETALA2-like gene from maize that regulates leaf epidermal cell identity. Genes & Dev., December 1, 1996; 10(23): 3018 - 3027. [Abstract] [PDF]

				X Li, Y Wu, D. Zhang, J. Gillikin, R. Boston, V. Franceschi, and T. Okita Rice prolamine protein body biogenesis: a BiP-mediated process Science, November 12, 1993; 262(5136): 1054 - 1056. [Abstract] [PDF]

				B. Satiat-Jeunemaitre and C. Hawes Redistribution of a Golgi glycoprotein in plant cells treated with Brefeldin A J. Cell Sci., December 1, 1992; 103(4): 1153 - 1166. [Abstract] [PDF]

				C. S. Kim, Y.-m. Woo, A. M. Clore, R. J. Burnett, N. P. Carneiro, and B. A. Larkins Zein Protein Interactions, Rather Than the Asymmetric Distribution of Zein mRNAs on Endoplasmic Reticulum Membranes, Influence Protein Body Formation in Maize Endosperm PLANT CELL, March 1, 2002; 14(3): 655 - 672. [Abstract] [Full Text] [PDF]

				Y. Takemoto, S. J. Coughlan, T. W. Okita, H. Satoh, M. Ogawa, and T. Kumamaru The Rice Mutant esp2 Greatly Accumulates the Glutelin Precursor and Deletes the Protein Disulfide Isomerase Plant Physiology, April 1, 2002; 128(4): 1212 - 1222. [Abstract] [Full Text] [PDF]