Plant Cell The Arabidopsis Book
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Staiger, C. J.
Right arrow Articles by Cande, W. Z.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Staiger, C. J.
Right arrow Articles by Cande, W. Z.
Agricola
Right arrow Articles by Staiger, C. J.
Right arrow Articles by Cande, W. Z.

THE PLANT CELL, Vol 3, Issue 6 637-644, Copyright © 1991 by American Society of Plant Biologists

RESEARCH ARTICLES

Microfilament Distribution in Maize Meiotic Mutants Correlates with Microtubule Organization

C. J. Staiger and W. Z. Cande
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California 94720

Microtubules and microfilaments often codistribute in plants; their presumed interaction can be tested with drugs although it is not always clear that these are without side effects. In this study, we exploited mutants defective in meiotic cell division to investigate in a noninvasive way the relationship between the two cytoskeletal elements. By staining unfixed, permeabilized cells with rhodamine-phalloidin, spatial and temporal changes in microfilament distribution during maize meiosis were examined. In wild-type microsporocytes, a microtubule array that radiates from the nucleus disappeared during spindle formation and returned at late telophase. This result differed from the complex cytoplasmic microfilament array that is present at all stages, including karyokinesis and cytokinesis. During division, a second class of microfilaments also was observed in the spindle and phragmoplast. To analyze this apparent association of microtubules and microfilaments, we examined several meiotic mutants known to have stage-specific disruptions in their microtubule arrays. Two mutations that altered the number or form of meiotic spindles also led to a dramatic reorganization of F-actin. In contrast, rearrangement of nonspindle, cytoplasmic microtubules did not lead to concomitant changes in F-actin distribution. These results suggested that microtubules and microfilaments interact in a cell cycle-specific and site-specific fashion during higher plant meiosis.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
L. U. Gilliland, M. K. Kandasamy, L. C. Pawloski, and R. B. Meagher
Both Vegetative and Reproductive Actin Isovariants Complement the Stunted Root Hair Phenotype of the Arabidopsis act2-1 Mutation
Plant Physiology, December 1, 2002; 130(4): 2199 - 2209.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J.-L. Magnard, M. Yang, Y.-C. S. Chen, M. Leary, and S. McCormick
The Arabidopsis Gene Tardy Asynchronous Meiosis Is Required for the Normal Pace and Synchrony of Cell Division during Male Meiosis
Plant Physiology, November 1, 2001; 127(3): 1157 - 1166.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. M. S. Smith and N. V. Raikhel
Nuclear Localization Signal Receptor Importin {alpha} Associates with the Cytoskeleton
PLANT CELL, November 1, 1998; 10(11): 1791 - 1800.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
Q Liu, I Golubovskaya, and W. Cande
Abnormal cytoskeletal and chromosome distribution in po, ms4 and ms6; mutant alleles of polymitotic that disrupt the cell cycle progression from meiosis to mitosis in maize
J. Cell Sci., January 12, 1993; 106(4): 1169 - 1178.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1991 by the American Society of Plant Biologists