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THE PLANT CELL, Vol 4, Issue 1 79-86, Copyright © 1992 by American Society of Plant Biologists
A Xanthomonas Pathogenicity Locus Is Induced by Sucrose and Sulfur-Containing Amino Acids
R. Schulte and U. Bonas
Institut fur Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, 1000 Berlin 33, Germany
Expression of hrp (hypersensitive reaction and pathogenicity) genes from
Xanthomonas campestris pv vesicatoria is suppressed in complex media but
induced in the plant. We examined the effects of macronutrients on
transcription of hrp-gusA ([beta]-glucuronidase) fusions by growth of the
bacteria in defined medium. Modified MM1 minimal medium, supplemented with
casamino acids, was able to induce hrpF strongly when sucrose or fructose
was added as a carbon source. However, high concentrations of casamino
acids suppressed hrpF induction. Sulfur-containing amino acids were
required for induction, with methionine induction being comparable to
induction in plants. Both sucrose and methionine were required for
induction. Induction in medium optimal for hrpF induction, designated XVM1,
occurred at pH 5.5 to pH 7.5. High concentrations of phosphate or sodium
chloride suppressed gene activation. Gene induction was inhibited by
succinate, citrate, pyruvate, and glutamine. Expression levels of different
hrp loci from X. c. vesicatoria in XVM1 varied, dependent on the genetic
background of the Xanthomonas strain used. The results suggest that several
control mechanisms might be involved in the expression of hrp genes.
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