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THE PLANT CELL, Vol 4, Issue 10 1237-1249, Copyright © 1992 by American Society of Plant Biologists
Ovule Development in Wild-Type Arabidopsis and Two Female-Sterile Mutants
K. Robinson-Beers, R. E. Pruitt and C. S. Gasser
Department of Biochemistry and Biophysics, University of California, Davis, California 95616
Ovules are complex structures that are present in all seed bearing plants
and are contained within the carpels in flowering plants. Ovules are the
site of megasporogenesis and megagametogenesis and, following
fertilization, develop into seeds. We combined genetic methods with
anatomical and morphological analyses to dissect ovule development. Here,
we present a detailed description of the morphological development of
Arabidopsis ovules and report on the isolation of two chemically induced
mutants, bell (bel1) and short integuments (sin1), with altered ovule
development. Phenotypic analyses indicated that bel1 mutants initiate a
single integument-like structure that develops aberrantly, sin1 mutants
initiate two integuments, but growth of the integuments is disrupted such
that cell division continues without normal cell elongation. Both mutants
can differentiate archesporial cells, but neither forms a normal embryo
sac. Genetic analyses indicated that bel1 segregates as a single recessive
mutation, and complementation tests showed that the two mutants are not
allelic. The phenotypes of the mutants indicate that normal morphological
development of the integuments and proper embryo sac formation are
interdependent or are governed in part by common pathways. The ovule
mutants that we describe in Arabidopsis represent novel genetic tools for
the study of this stage of reproductive development.
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