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THE PLANT CELL, Vol 4, Issue 12 1495-1505, Copyright © 1992 by American Society of Plant Biologists
Transgenic Maize Plants by Tissue Electroporation
K. D'Halluin, E. Bonne, M. Bossut, M. De Beuckeleer and J. Leemans
Plant Genetic Systems N.V., J. Plateaustraat 22, B-9000 Gent, Belgium
In this paper, we describe the transformation of regenerable maize tissues
by electroporation. In many maize lines, immature zygotic embryos can give
rise to embryogenic callus cultures from which plants can be regenerated.
Immature zygotic embryos or embryogenic type I calli were wounded either
enzymatically or mechanically and subsequently electroporated with a
chimeric gene encoding neomycin phosphotransferase (neo). Transformed
embryogenic calli were selected from electroporated tissues on
kanamycin-containing media and fertile transgenic maize plants were
regenerated. The neo gene was transmitted to the progeny of
kanamycin-resistant transformants in a Mendelian fashion. This showed that
all transformants were nonchimeric, suggesting that transformation and
regeneration are a single-cell event. The maize transformation procedure
presented here does not require the establishment of genotype-dependent
embryogenic type II callus or cell suspension cultures and facilitates the
engineering of new traits into agronomically relevant maize inbred lines.
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