|
THE PLANT CELL, Vol 4, Issue 12 1531-1538, Copyright © 1992 by American Society of Plant Biologists
Alfalfa Cyclins: Differential Expression during the Cell Cycle and in Plant Organs
H. Hirt, M. Mink, M. Pfosser, L. Bogre, J. Gyorgyey, C. Jonak, A. Gartner, D. Dudits and E. Heberle-Bors
Institute of Microbiology and Genetics, University of Vienna, Vienna Biocenter, Dr. Bohrgasse 9, 1030 Vienna, Austria
Cell division in eukaryotes is mediated by the action of the mitosis
promoting factor, which is composed of the CDC2 protein kinase and one of
the various mitotic cyclins. We have recently isolated a cdc2 gene from
alfalfa. Here, we report the isolation of two cyclin genes, cycMs1 and
cycMs2, from alfalfa. The cycMs2 gene shows highest similarity to type B
cyclins. In contrast, the predicted amino acid sequence of the cycMs1 gene
shows similar homology scores to cyclins of all types (25 to 35%). Both
genes are expressed in dividing suspension cultured cells but cease to be
expressed when the cells enter stationary phase. In synchronized alfalfa
suspension cultured cells, the mRNAs of cycMs1 and cycMs2 show maximal
expression in the G2 and M phases. Transcripts of cycMs2 are found only in
late G2 and M phase cells, an expression pattern typical for cyclin B
genes, whereas cycMs1 appears with the onset of G2. This pattern indicates
that alfalfa cycMs1 and cycMs2 belong to different classes of cyclins. In
young leaves, expression of both genes is high, whereas in mature leaves no
transcripts can be detected, indicating that the two cyclin genes are true
cell division markers at the mRNA level. In other organs, a more complex
expression pattern of the two cyclin genes was found.
This article has been cited by other articles:
|
|
|
|
 
M. Weingartner, H. R. Pelayo, P. Binarova, K. Zwerger, B. Melikant, C. de la Torre, E. Heberle-Bors, and L. Bogre
A plant cyclin B2 is degraded early in mitosis and its ectopic expression shortens G2-phase and alleviates the DNA-damage checkpoint
J. Cell Sci.,
February 1, 2003;
116(3):
487 - 498.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Larkins, B. P. Dilkes, R. A. Dante, C. M. Coelho, Y.-m. Woo, and Y. Liu
Investigating the hows and whys of DNA endoreduplication
J. Exp. Bot.,
February 1, 2001;
52(355):
183 - 192.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Bögre, O. Calderini, P. Binarova, M. Mattauch, S. Till, S. Kiegerl, C. Jonak, C. Pollaschek, P. Barker, N. S. Huskisson, et al.
A MAP Kinase Is Activated Late in Plant Mitosis and Becomes Localized to the Plane of Cell Division
PLANT CELL,
January 1, 1999;
11(1):
101 - 114.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. A. Sorrell, B. Combettes, N. Chaubet-Gigot, C. Gigot, and J. A.H. Murray
Distinct Cyclin D Genes Show Mitotic Accumulation or Constant Levels of Transcripts in Tobacco Bright Yellow-2 Cells
Plant Physiology,
January 1, 1999;
119(1):
343 - 352.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Ito, M. Iwase, H. Kodama, P. Lavisse, A. Komamine, R. Nishihama, Y. Machida, and A. Watanabe
A Novel cis-Acting Element in Promoters of Plant B-Type Cyclin Genes Activates M Phase – Specific Transcription
PLANT CELL,
March 1, 1998;
10(3):
331 - 342.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hirayama and K. Shinozaki
A cdc5+ homolog of a higher plant, Arabidopsis thaliana
PNAS,
November 12, 1996;
93(23):
13371 - 13376.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
