|
THE PLANT CELL, Vol 4, Issue 12 1539-1548, Copyright © 1992 by American Society of Plant Biologists
Pumpkin Phloem Lectin Genes Are Specifically Expressed in Companion Cells
D. E. Bostwick, J. M. Dannenhoffer, M. I. Skaggs, R. M. Lister, B. A. Larkins and G. A. Thompson
Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721
Pumpkin phloem exudate contains two abundant phloem proteins: PP1 is a
96-kD protein that forms polymeric filaments in vivo, and PP2 is a 48-kD
dimeric lectin. Polyclonal antibodies raised against pumpkin phloem exudate
were used to isolate several cDNAs corresponding to PP1 and PP2. RNA gel
blot analysis indicated that PP1 is encoded by an mRNA of ~2500
nucleotides, whereas PP2 subunits are encoded by an mRNA of 1000
nucleotides. Sequence analysis of PP2 cDNAs revealed a 654-bp open reading
frame encoding a 218-amino acid polypeptide; this polypeptide had the
carbohydrate binding characteristics of a PP2 subunit. The PP2 mRNA was
localized within the phloem of pumpkin hypocotyl cross-sections based on in
situ hybridization of a digoxigenin-labeled antisense probe. PP2 mRNA was
found within the companion cells in both the bicollateral vascular bundles
and the extrafascicular phloem network.
This article has been cited by other articles:
|
|
|
|
 
H. C. Pelissier, W. S. Peters, R. Collier, A. J. E. v. Bel, and M. Knoblauch
GFP Tagging of Sieve Element Occlusion (SEO) Proteins Results in Green Fluorescent Forisomes
Plant Cell Physiol.,
November 1, 2008;
49(11):
1699 - 1710.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Suzuki, M. Yamamura, T. Hattori, T. Nakatsubo, and T. Umezawa
The subunit composition of hinokiresinol synthase controls geometrical selectivity in norlignan formation
PNAS,
December 26, 2007;
104(52):
21008 - 21013.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Will and A. J. E. van Bel
Physical and chemical interactions between aphids and plants
J. Exp. Bot.,
March 1, 2006;
57(4):
729 - 737.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kehr
Phloem sap proteins: their identities and potential roles in the interaction between plants and phloem-feeding insects
J. Exp. Bot.,
March 1, 2006;
57(4):
767 - 774.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. l. C. Petersen, J. Hejgaard, G. A. Thompson, and A. Schulz
Cucurbit phloem serpins are graft-transmissible and appear to be resistant to turnover in the sieve element-companion cell complex
J. Exp. Bot.,
December 1, 2005;
56(422):
3111 - 3120.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Fukuda, S. Fujimaki, T. Mori, N. Suzui, K. Ishiyama, T. Hayakawa, T. Yamaya, T. Fujiwara, T. Yoneyama, and H. Hayashi
Differential Distribution of Proteins Expressed in Companion Cells in the Sieve Element-Companion Cell Complex of Rice Plants
Plant Cell Physiol.,
November 1, 2005;
46(11):
1779 - 1786.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Vignault, M. Vachaud, B. Cakir, D. Glissant, F. Dedaldechamp, M. Buttner, R. Atanassova, P. Fleurat-Lessard, R. Lemoine, and S. Delrot
VvHT1 encodes a monosaccharide transporter expressed in the conducting complex of the grape berry phloem
J. Exp. Bot.,
May 1, 2005;
56(415):
1409 - 1418.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Gomez and V. Pallas
A Long-Distance Translocatable Phloem Protein from Cucumber Forms a Ribonucleoprotein Complex In Vivo with Hop Stunt Viroid RNA
J. Virol.,
September 15, 2004;
78(18):
10104 - 10110.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Bird, V. R. Franceschi, and P. J. Facchini
A Tale of Three Cell Types: Alkaloid Biosynthesis Is Localized to Sieve Elements in Opium Poppy
PLANT CELL,
November 1, 2003;
15(11):
2626 - 2635.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Dinant, A. M. Clark, Y. Zhu, F. Vilaine, J.-C. Palauqui, C. Kusiak, and G. A. Thompson
Diversity of the Superfamily of Phloem Lectins (Phloem Protein 2) in Angiosperms
Plant Physiology,
January 1, 2003;
131(1):
114 - 128.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Haywood, F. Kragler, and W. J. Lucas
Plasmodesmata: Pathways for Protein and Ribonucleoprotein Signaling
PLANT CELL,
May 1, 2002;
14(90001):
S303 - 325.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Imlau, E. Truernit, and N. Sauer
Cell-to-Cell and Long-Distance Trafficking of the Green Fluorescent Protein in the Phloem and Symplastic Unloading of the Protein into Sink Tissues
PLANT CELL,
March 1, 1999;
11(3):
309 - 322.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R Ruiz-Medrano, B Xoconostle-Cazares, and W. Lucas
Phloem long-distance transport of CmNACP mRNA: implications for supracellular regulation in plants
Development,
January 10, 1999;
126(20):
4405 - 4419.
[Abstract]
[PDF]
|
|
|
|
|
|
|
B. Golecki, A. Schulz, and G. A. Thompson
Translocation of Structural P Proteins in the Phloem
PLANT CELL,
January 1, 1999;
11(1):
127 - 140.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Itaya, Y.-M. Woo, C. Masuta, Y. Bao, R. S. Nelson, and B. Ding
Developmental Regulation of Intercellular Protein Trafficking through Plasmodesmata in Tobacco Leaf Epidermis
Plant Physiology,
October 1, 1998;
118(2):
373 - 385.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Balachandran, Y. Xiang, C. Schobert, G. A. Thompson, and W. J. Lucas
Phloem sap proteins from Cucurbita maxima and Ricinus communis have the capacity to traffic cell to cell through plasmodesmata
PNAS,
December 9, 1997;
94(25):
14150 - 14155.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Kühn, V. R. Franceschi, A. Schulz, R. Lemoine, and W. B. Frommer
Macromolecular Trafficking Indicated by Localization and Turnover of Sucrose Transporters in Enucleate Sieve Elements
Science,
February 28, 1997;
275(5304):
1298 - 1300.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. S. Kim, Y.-m. Woo, A. M. Clore, R. J. Burnett, N. P. Carneiro, and B. A. Larkins
Zein Protein Interactions, Rather Than the Asymmetric Distribution of Zein mRNAs on Endoplasmic Reticulum Membranes, Influence Protein Body Formation in Maize Endosperm
PLANT CELL,
March 1, 2002;
14(3):
655 - 672.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
