Plant Cell Plant Cell 20th
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (68)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Grimes, H. D.
Right arrow Articles by Hitz, W. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Grimes, H. D.
Right arrow Articles by Hitz, W. D.
Agricola
Right arrow Articles by Grimes, H. D.
Right arrow Articles by Hitz, W. D.

THE PLANT CELL, Vol 4, Issue 12 1561-1574, Copyright © 1992 by American Society of Plant Biologists


RESEARCH ARTICLES

A 62-kD Sucrose Binding Protein Is Expressed and Localized in Tissues Actively Engaged in Sucrose Transport

H. D. Grimes, P. J. Overvoorde, K. Ripp, V. R. Franceschi and W. D. Hitz
Department of Botany, Washington State University, Pullman, Washington 99164-4238

Sucrose transport from the apoplasm, across the plasma membrane, and into the symplast is critical for growth and development in most plant species. Phloem loading, the process of transporting sucrose against a concentration gradient into the phloem, is an essential first step in long-distance transport of sucrose and carbon partitioning. We report here that a soybean 62-kD sucrose binding protein is associated with the plasma membrane of several cell types engaged in sucrose transport, including the mesophyll cells of young sink leaves, the companion cells of mature phloem, and the cells of the developing cotyledons. Furthermore, the temporal expression of the gene and the accumulation pattern of the protein closely parallel the rate of sucrose uptake in the cotyledon. Molecular cloning and sequence analysis of a full-length cDNA for this 62-kD sucrose binding protein indicated that the protein is not an invertase, contains a 29-amino acid leader peptide that is absent from the mature protein, and is not an integral membrane protein. We conclude that the 62-kD sucrose binding protein is involved in sucrose transport, but is not performing this function independently.


This article has been cited by other articles:


Home page
Crop Sci.Home page
C. Ren, K. D. Bilyeu, and P. R. Beuselinck
Composition, Vigor, and Proteome of Mature Soybean Seeds Developed under High Temperature
Crop Sci., May 11, 2009; 49(3): 1010 - 1022.
[Abstract] [Full Text] [PDF]


Home page
J Exp BotHome page
J. Wang, P. K. Suen, Z.-F. Xu, and L. Jiang
A 64 kDa sucrose binding protein is membrane-associated and tonoplast-localized in developing mung bean seeds
J. Exp. Bot., February 1, 2009; 60(2): 629 - 639.
[Abstract] [Full Text] [PDF]


Home page
J Exp BotHome page
J. Castillo, A. Genoves, L. Franco, and M. I. Rodrigo
A multifunctional bicupin serves as precursor for a chromosomal protein of Pisum sativum seeds
J. Exp. Bot., December 1, 2005; 56(422): 3159 - 3169.
[Abstract] [Full Text] [PDF]


Home page
Plant Physiol.Home page
M. Hajduch, A. Ganapathy, J. W. Stein, and J. J. Thelen
A Systematic Proteomic Study of Seed Filling in Soybean. Establishment of High-Resolution Two-Dimensional Reference Maps, Expression Profiles, and an Interactive Proteome Database
Plant Physiology, April 1, 2005; 137(4): 1397 - 1419.
[Abstract] [Full Text] [PDF]


Home page
J Exp BotHome page
L. A. S. Contim, A. J. Waclawovsky, N. Delu-Filho, C. P. Pirovani, W. R. Clarindo, M. E. Loureiro, C. R. Carvalho, and E. P. B. Fontes
The soybean sucrose binding protein gene family: genomic organization, gene copy number and tissue-specific expression of the SBP2 promoter
J. Exp. Bot., December 1, 2003; 54(393): 2643 - 2653.
[Abstract] [Full Text] [PDF]


Home page
J Exp BotHome page
A. de Jong and A.C. Borstlap
A plasma membrane-enriched fraction isolated from the coats of developing pea seeds contains H+-symporters for amino acids and sucrose
J. Exp. Bot., October 1, 2000; 51(351): 1671 - 1677.
[Abstract] [Full Text] [PDF]


Home page
Microbiol. Mol. Biol. Rev.Home page
J. M. Dunwell, S. Khuri, and P. J. Gane
Microbial Relatives of the Seed Storage Proteins of Higher Plants: Conservation of Structure and Diversification of Function during Evolution of the Cupin Superfamily
Microbiol. Mol. Biol. Rev., March 1, 2000; 64(1): 153 - 179.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
P. J. Overvoorde, W. S. Chao, and H. D. Grimes
A Plasma Membrane Sucrose-binding Protein That Mediates Sucrose Uptake Shares Structural and Sequence Similarity with Seed Storage Proteins but Remains Functionally Distinct
J. Biol. Chem., June 20, 1997; 272(25): 15898 - 15904.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1992 by the American Society of Plant Biologists