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THE PLANT CELL, Vol 4, Issue 2 149-159, Copyright © 1992 by American Society of Plant Biologists
A Meristem-Related Gene from Tomato Encodes a dUTPase: Analysis of Expression in Vegetative and Floral Meristems
A. Pri-Hadash, D. Hareven and E. Lifschitz
Department of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel
A meristem-specific gene coding for deoxyuridine triphosphatase (EC
3.6.1.23) (dUTPase) in tomato was isolated, and its developmental
expression in vegetative and floral apices was monitored. An 18-kD
polypeptide, P18, was isolated as a consequence of its accumulation in
arrested floral meristems of anantha mutant plants. The corresponding cDNA
isolated from an expression library exhibited a 40 to 60% similarity with
the pseudoprotease sequences of poxviruses, genes that have been suggested
to encode dUTPases. Enzymatic tests and conservation of peptide motifs
common to bacterial and viral genes verified that the P18 cDNA clone indeed
represents a eukaryotic dUTPase. Immunogold localization and in situ
hybridization experiments showed that polypeptides and transcripts of
dUTPase are maintained at high levels in apical meristematic cells of
vegetative and floral meristems. dUTPase gene activity is also high in the
potentially meristematic cells of the provascular and vascular system. Its
expression is lower in the immediate parenchymal derivatives of the apical
meristematic cells, and this downregulation marks, perhaps, the transition
from totipotency to the first differentiated state.
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