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THE PLANT CELL, Vol 4, Issue 5 539-547, Copyright © 1992 by American Society of Plant Biologists
The Small Genome of Arabidopsis Contains at Least Six Expressed [alpha]-Tubulin Genes
S. D. Kopczak, N. A. Haas, P. J. Hussey, C. D. Silflow and D. P. Snustad
Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55108-1095
The goal of our investigations is to define the genetic control of
microtubule-based processes in a higher plant. The available evidence
suggests that we have achieved our first objective: the characterization of
the complete [alpha]-tubulin and [beta]-tubulin gene families of
Arabidopsis. Four additional [alpha]-tubulin genes (TUA2, TUA4, TUA5, and
TUA6) of Arabidopsis have been cloned and sequenced to complete the
analysis of the gene structure for all six [alpha]-tubulin genes detectable
on DNA gel blots of Arabidopsis genomic DNA hybridized with [alpha]-tubulin
coding sequences. TUA1 and TUA3 were characterized earlier in our
laboratory. Noncoding gene-specific hybridization probes have been
constructed for all six [alpha]-tubulin genes and used in RNA gel blot
analyses to demonstrate that all six genes are transcribed. The six genes
encode four different [alpha]-tubulin isoforms; TUA2 and TUA4 encode a
single isoform, as do TUA3 and TUA5. Two-dimensional protein gel immunoblot
analyses have resolved at least four [alpha]-tubulin isoforms from plant
tissues, suggesting that all of the predicted TUA gene products are
synthesized in vivo.
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