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THE PLANT CELL, Vol 4, Issue 5 557-571, Copyright © 1992 by American Society of Plant Biologists
Preferential Expression of an [alpha]-Tubulin Gene of Arabidopsis in Pollen
J. L. Carpenter, S. E. Ploense, D. P. Snustad and C. D. Silflow
Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55108-1095
The pool of tubulin protein in tissues of Arabidopsis is provided by the
expression of multiple [alpha]-tubulin (TUA) and [beta]-tubulin genes.
Whereas most tubulin genes are expressed in many tissues, previous evidence
suggested that the TUA1 gene might be expressed primarily in pollen. We now
report a detailed analysis of TUA1 expression during Arabidopsis
development. In RNA from tissues of dissected flowers, TUA1 transcripts
were detected only in stamens and mature pollen. Chimeric genes containing
TUA1 5[prime] flanking DNA fused to the [beta]-glucuronidase (GUS) coding
region were used to create transgenic Arabidopsis plants. Plants containing
a chimeric gene with 533 bp of 5[prime] flanking sequence were analyzed by
histochemical assay to localize GUS expression within the plant. The blue
product of GUS enzyme activity accumulated very rapidly in postmitotic
pollen grains. Much lower levels of GUS activity were detected in anthers
with uninucleate pollen grains, in flower receptacles, and in a few
vegetative tissues. Analysis of 5[prime] deletions of the TUA1 promoter
suggested that 97 bp of 5[prime] flanking DNA is sufficient to drive GUS
expression in pollen and young anthers, whereas at least 380 bp is required
to detect GUS expression in the receptacle. Examination of the TUA1
promoter sequence revealed several motifs that are repeated within the TUA1
promoter and are similar to sequences in other pollen-specific promoters.
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