THE PLANT CELL, Vol 4, Issue 9 1053-1062, Copyright © 1992 by American Society of Plant Biologists
Specific Expression of an Extensin-Like Gene in the Style of Nicotiana alata
C. G. Chen, E. C. Cornish and A. E. Clarke
Plant Cell Biology Research Centre, University of Melbourne, Parkville, Victoria 3052, Australia
cDNAs and corresponding genomic clones encoding a putative proline-rich
protein (NaPRP3) were isolated from libraries prepared from Nicotiana alata
style mRNA and genomic DNA. The predicted NaPRP3 protein is structurally
similar to extensin in containing six copies of the characteristic extensin
sequence Ser-Pro4, but differs in being smaller (151 residues compared with
>300 residues) and lacking Tyr residues. In contrast to most extensin
genes, the NaPRP3 gene is not induced by mechanical wounding, and its
expression is restricted to cells of the transmitting tract of the style.