Plant Cell Hybrigenics The Protein Interactions Experts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (220)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Millar, A. J.
Right arrow Articles by Kay, S. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Millar, A. J.
Right arrow Articles by Kay, S. A.
Agricola
Right arrow Articles by Millar, A. J.
Right arrow Articles by Kay, S. A.

THE PLANT CELL, Vol 4, Issue 9 1075-1087, Copyright © 1992 by American Society of Plant Biologists

RESEARCH ARTICLES

A Novel Circadian Phenotype Based on Firefly Luciferase Expression in Transgenic Plants

A. J. Millar, S. R. Short, N. H. Chua and S. A. Kay
Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, New York, New York 10021-6399

A 320-bp fragment of the Arabidopsis cab2 promoter is sufficient to mediate transcriptional regulation by both phytochrome and the circadian clock. We fused this promoter fragment to the firefly luciferase (Luc) gene to create a real-time reporter for regulated gene expression in intact plants. Cab2::Luc transcript accumulated in the expected patterns and luciferase activity was closely correlated to cab2::Luc mRNA abundance in both etiolated and green seedlings. The concentration of the bulk of luciferase protein did not reflect these patterns but maintained a relatively constant level, implying that a post-translational mechanism(s) leads to the high-amplitude regulation of luciferase activity. We used a low-light video imaging system to establish that luciferase bioluminescence in vivo accurately reports the temporal and spatial regulation of cab2 transcription in single seedlings. The unique qualities of the firefly luciferase system allowed us to monitor regulated gene expression in real time in individual multicellular organisms. This noninvasive marker for temporal regulation at the molecular level constitutes a circadian phenotype, which may be used to isolate mutants in the circadian clock.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
S. X. Lu, S. M. Knowles, C. Andronis, M. S. Ong, and E. M. Tobin
CIRCADIAN CLOCK ASSOCIATED1 and LATE ELONGATED HYPOCOTYL Function Synergistically in the Circadian Clock of Arabidopsis
Plant Physiology, June 1, 2009; 150(2): 834 - 843.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. B. Robertson, C. C. Stowers, E. Boczko, and C. Hirschie Johnson
Real-time luminescence monitoring of cell-cycle and respiratory oscillations in yeast
PNAS, November 18, 2008; 105(46): 17988 - 17993.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. A. Salome, Q. Xie, and C. R. McClung
Circadian Timekeeping during Early Arabidopsis Development
Plant Physiology, July 1, 2008; 147(3): 1110 - 1125.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
V. D. Gooch, A. Mehra, L. F. Larrondo, J. Fox, M. Touroutoutoudis, J. J. Loros, and J. C. Dunlap
Fully Codon-Optimized luciferase Uncovers Novel Temperature Characteristics of the Neurospora Clock
Eukaryot. Cell, January 1, 2008; 7(1): 28 - 37.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
J. Koo, Y. Kim, J. Kim, M. Yeom, I. C. Lee, and H. G. Nam
A GUS/Luciferase Fusion Reporter for Plant Gene Trapping and for Assay of Promoter Activity with Luciferin-Dependent Control of the Reporter Protein Stability
Plant Cell Physiol., August 1, 2007; 48(8): 1121 - 1131.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Perales and P. Mas
A Functional Link between Rhythmic Changes in Chromatin Structure and the Arabidopsis Biological Clock
PLANT CELL, July 1, 2007; 19(7): 2111 - 2123.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Moulager, A. Monnier, B. Jesson, R. Bouvet, J. Mosser, C. Schwartz, L. Garnier, F. Corellou, and F.-Y. Bouget
Light-Dependent Regulation of Cell Division in Ostreococcus: Evidence for a Major Transcriptional Input
Plant Physiology, July 1, 2007; 144(3): 1360 - 1369.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. G. McWatters, E. Kolmos, A. Hall, M. R. Doyle, R. M. Amasino, P. Gyula, F. Nagy, A. J. Millar, and S. J. Davis
ELF4 Is Required for Oscillatory Properties of the Circadian Clock
Plant Physiology, May 1, 2007; 144(1): 391 - 401.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
S. S. Golden
Integrating the Circadian Oscillator into the Life of the Cyanobacterial Cell
Cold Spring Harb Symp Quant Biol, January 1, 2007; 72(0): 331 - 338.
[Abstract] [PDF]

Home page
 Plant Physiol.Home page
C. B. Engineer and R. G. Kranz
Reciprocal Leaf and Root Expression of AtAmt1.1 and Root Architectural Changes in Response to Nitrogen Starvation
Plant Physiology, January 1, 2007; 143(1): 236 - 250.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
T. Allen, A. Koustenis, G. Theodorou, D. E. Somers, S. A. Kay, G. C. Whitelam, and P. F. Devlin
Arabidopsis FHY3 Specifically Gates Phytochrome Signaling to the Circadian Clock
PLANT CELL, October 1, 2006; 18(10): 2506 - 2516.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. I. A. Calderon-Villalobos, C. Kuhnle, H. Li, M. Rosso, B. Weisshaar, and C. Schwechheimer
LucTrap Vectors Are Tools to Generate Luciferase Fusions for the Quantification of Transcript and Protein Abundance in Vivo.
Plant Physiology, May 1, 2006; 141(1): 3 - 14.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
C. R. McClung
Plant circadian rhythms.
PLANT CELL, April 1, 2006; 18(4): 792 - 803.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Darrah, B. L. Taylor, K. D. Edwards, P. E. Brown, A. Hall, and H. G. McWatters
Analysis of Phase of LUCIFERASE Expression Reveals Novel Circadian Quantitative Trait Loci in Arabidopsis
Plant Physiology, April 1, 2006; 140(4): 1464 - 1474.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
G. An, S. Lee, S.-H. Kim, and S.-R. Kim
Molecular Genetics Using T-DNA in Rice
Plant Cell Physiol., January 15, 2005; 46(1): 14 - 22.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Christmann, T. Hoffmann, I. Teplova, E. Grill, and A. Muller
Generation of Active Pools of Abscisic Acid Revealed by In Vivo Imaging of Water-Stressed Arabidopsis
Plant Physiology, January 1, 2005; 137(1): 209 - 219.
[Abstract] [Full Text] [PDF]

Home page
 J Biol RhythmsHome page
J. S. Takahashi
Finding New Clock Components: Past and Future
J Biol Rhythms, October 1, 2004; 19(5): 339 - 347.
[Abstract] [PDF]

Home page
 J Biol RhythmsHome page
P. A. Salome and C. R. McClung
The Arabidopsis thaliana Clock
J Biol Rhythms, October 1, 2004; 19(5): 425 - 435.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
S. V. Markova, S. Golz, L. A. Frank, B. Kalthof, and E. S. Vysotski
Cloning and Expression of cDNA for a Luciferase from the Marine Copepod Metridia longa: A NOVEL SECRETED BIOLUMINESCENT REPORTER ENZYME
J. Biol. Chem., January 30, 2004; 279(5): 3212 - 3217.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. C. Alvarado, L. M. Zsigmond, I. Kovacs, A. Cseplo, C. Koncz, and L. M. Szabados
Gene Trapping with Firefly Luciferase in Arabidopsis. Tagging of Stress-Responsive Genes
Plant Physiology, January 1, 2004; 134(1): 18 - 27.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. P. Michael and C. R. McClung
Phase-Specific Circadian Clock Regulatory Elements in Arabidopsis
Plant Physiology, October 1, 2002; 130(2): 627 - 638.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. A. Iliev, W. Xu, D. H. Polisensky, M.-H. Oh, R. S. Torisky, S. D. Clouse, and J. Braam
Transcriptional and Posttranscriptional Regulation of Arabidopsis TCH4 Expression by Diverse Stimuli. Roles of cis Regions and Brassinosteroids
Plant Physiology, October 1, 2002; 130(2): 770 - 783.
[Abstract] [Full Text] [PDF]

Home page
 Sci SignalHome page
V. Chinnusamy, B. Stevenson, B.-h. Lee, and J.-K. Zhu
Screening for Gene Regulation Mutants by Bioluminescence Imaging
Sci. Signal., July 9, 2002; 2002(140): pl10 - pl10.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. F. Devlin
Signs of the time: environmental input to the circadian clock
J. Exp. Bot., July 1, 2002; 53(374): 1535 - 1550.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
I. A. Carre and J.-Y. Kim
MYB transcription factors in the Arabidopsis circadian clock
J. Exp. Bot., July 1, 2002; 53(374): 1551 - 1557.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. Toth, E. Kevei, A. Hall, A. J. Millar, F. Nagy, and L. Kozma-Bognar
Circadian Clock-Regulated Expression of Phytochrome and Cryptochrome Genes in Arabidopsis
Plant Physiology, December 1, 2001; 127(4): 1607 - 1616.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Hall, L. Kozma-Bognar, R. Toth, F. Nagy, and A. J. Millar
Conditional Circadian Regulation of PHYTOCHROME A Gene Expression
Plant Physiology, December 1, 2001; 127(4): 1808 - 1818.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. F. Covington, S. Panda, X. L. Liu, C. A. Strayer, D. R. Wagner, and S. A. Kay
ELF3 Modulates Resetting of the Circadian Clock in Arabidopsis
PLANT CELL, June 1, 2001; 13(6): 1305 - 1316.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Y. Xu and C. H. Johnson
A Clock- and Light-Regulated Gene That Links the Circadian Oscillator to LHCB Gene Expression
PLANT CELL, June 1, 2001; 13(6): 1411 - 1426.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. S. Golden and C. Strayer
Time for Plants. Progress in Plant Chronobiology
Plant Physiology, January 1, 2001; 125(1): 98 - 101.
[Full Text]

Home page
 Genes Dev.Home page
Q. Xie, G. Frugis, D. Colgan, and N.-H. Chua
Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development
Genes & Dev., December 1, 2000; 14(23): 3024 - 3036.
[Abstract] [Full Text]

Home page
 Plant CellHome page
P. F. Devlin and S. A. Kay
Cryptochromes Are Required for Phytochrome Signaling to the Circadian Clock but Not for Rhythmicity
PLANT CELL, December 1, 2000; 12(12): 2499 - 2510.
[Abstract] [Full Text]

Home page
 Plant CellHome page
L. Loyall, K. Uchida, S. Braun, M. Furuya, and H. Frohnmeyer
Glutathione and a UV Light-Induced Glutathione S-Transferase Are Involved in Signaling to Chalcone Synthase in Cell Cultures
PLANT CELL, October 1, 2000; 12(10): 1939 - 1950.
[Abstract] [Full Text]

Home page
 J Biol RhythmsHome page
J. A. Kreps, T. Muramatsu, M. Furuya, and S. A. Kay
Fluorescent Differential Display Identifies Circadian Clock-Regulated Genes in Arabidopsis thaliana
J Biol Rhythms, June 1, 2000; 15(3): 208 - 217.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. K. Bognar, A. Hall, E. Adam, S. C. Thain, F. Nagy, and A. J. Millar
The circadian clock controls the expression pattern of the circadian input photoreceptor, phytochrome B
PNAS, December 7, 1999; 96(25): 14652 - 14657.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Sai and C. H. Johnson
Different circadian oscillators control Ca2+ fluxes and Lhcb gene expression
PNAS, September 28, 1999; 96(20): 11659 - 11663.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
M. Katayama, N. F. Tsinoremas, T. Kondo, and S. S. Golden
cpmA, a Gene Involved in an Output Pathway of the Cyanobacterial Circadian System
J. Bacteriol., June 1, 1999; 181(11): 3516 - 3524.
[Abstract] [Full Text]

Home page
 JCBHome page
R. Yamaguchi, M. Nakamura, N. Mochizuki, S. A. Kay, and A. Nagatani
Light-dependent Translocation of a Phytochrome B-GFP Fusion Protein to the Nucleus in Transgenic Arabidopsis
J. Cell Biol., May 3, 1999; 145(3): 437 - 445.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Hondred, J. M. Walker, D. E. Mathews, and R. D. Vierstra
Use of Ubiquitin Fusions to Augment Protein Expression in Transgenic Plants
Plant Physiology, February 1, 1999; 119(2): 713 - 724.
[Abstract] [Full Text]

Home page
 DevelopmentHome page
C Steindler, A Matteucci, G Sessa, T Weimar, M Ohgishi, T Aoyama, G Morelli, and I Ruberti
Shade avoidance responses are mediated by the ATHB-2 HD-zip protein, a negative regulator of gene expression
Development, January 10, 1999; 126(19): 4235 - 4245.
[Abstract] [PDF]

Home page
 Plant Physiol.Home page
L. Xiong, M. Ishitani, and J.-K. Zhu
Interaction of Osmotic Stress, Temperature, and Abscisic Acid in the Regulation of Gene Expression in Arabidopsis
Plant Physiology, January 1, 1999; 119(1): 205 - 212.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
M. L. Salvador, U. Klein, and L. Bogorad
Endogenous Fluctuations of DNA Topology in the Chloroplast of Chlamydomonas reinhardtii
Mol. Cell. Biol., December 1, 1998; 18(12): 7235 - 7242.
[Abstract] [Full Text]

Home page
 Plant CellHome page
H. H. Zhong, J. E. Painter, P. A. Salomé, M. Straume, and C. R. McClung
Imbibition, but Not Release from Stratification, Sets the Circadian Clock in Arabidopsis Seedlings
PLANT CELL, December 1, 1998; 10(12): 2005 - 2018.
[Abstract] [Full Text]

Home page
 ScienceHome page
D. E. Somers, P. F. Devlin, and S. A. Kay
Phytochromes and Cryptochromes in the Entrainment of the Arabidopsis Circadian Clock
Science, November 20, 1998; 282(5393): 1488 - 1490.
[Abstract] [Full Text]

Home page
 ScienceHome page
M. Ishiura, S. Kutsuna, S. Aoki, H. Iwasaki, C. R. Andersson, A. Tanabe, S. S. Golden, C. H. Johnson, and T. Kondo
Expression of a Gene Cluster kaiABC as a Circadian Feedback Process in Cyanobacteria
Science, September 4, 1998; 281(5382): 1519 - 1523.
[Abstract] [Full Text]

Home page
 Plant CellHome page
T. Genoud, A. J. Millar, N. Nishizawa, S. A. Kay, E. Schäfer, A. Nagatani, and N.-H. Chua
An Arabidopsis Mutant Hypersensitive to Red and Far-Red Light Signals
PLANT CELL, June 1, 1998; 10(6): 889 - 904.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
N. Takasuka, M. R. H. White, C. D. Wood, W. R. Robertson, and J. R. E. Davis
Dynamic Changes in Prolactin Promoter Activation in Individual Living Lactotrophic Cells
Endocrinology, March 1, 1998; 139(3): 1361 - 1368.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
D. Somers, A. Webb, M Pearson, and S. Kay
The short-period mutant, toc1-1, alters circadian clock regulation of multiple outputs throughout development in Arabidopsis thaliana
Development, January 2, 1998; 125(3): 485 - 494.
[Abstract] [PDF]

Home page
 ScienceHome page
Y. Wu, J. Kuzma, E. Maréchal, R. Graeff, H. C. Lee, R. Foster, and N. Chua
Abscisic Acid Signaling Through Cyclic ADP-Ribose in Plants
Science, December 19, 1997; 278(5346): 2126 - 2130.
[Abstract] [Full Text]

Home page
 J Biol RhythmsHome page
J. D. Plautz, M. Straume, R. Stanewsky, C. F. Jamison, C. Brandes, H. B. Dowse, J. C. Hall, and S. A. Kay
Quantitative Analysis of Drosophila period Gene Transcription in Living Animals
J Biol Rhythms, June 1, 1997; 12(3): 204 - 217.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. J. Millar and S. A. Kay
Integration of circadian and phototransduction pathways in the network controlling CAB gene transcription in Arabidopsis
PNAS, December 24, 1996; 93(26): 15491 - 15496.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
K. A. Hicks, A. J. Millar, I. A. Carré, D. E. Somers, M. Straume, D. R. Meeks-Wagner, and S. A. Kay
Conditional Circadian Dysfunction of the Arabidopsis early-flowering 3 Mutant
Science, November 1, 1996; 274(5288): 790 - 792.
[Abstract] [Full Text]

Home page
 J Biol RhythmsHome page
P. Markovic, T. Roenneberg, and D. Morse
Phased Protein Synthesis at Several Circadian Times Does Not Change Protein Levels in Gonyaulax
J Biol Rhythms, March 1, 1996; 11(1): 57 - 67.
[Abstract] [PDF]

Home page
 ScienceHome page
C. Johnson, M. Knight, T Kondo, P Masson, J Sedbrook, A Haley, and A Trewavas
Circadian oscillations of cytosolic and chloroplastic free calcium in plants
Science, September 29, 1995; 269(5232): 1863 - 1865.
[Abstract] [PDF]

Home page
 ScienceHome page
A. Millar, I. Carre, C. Strayer, N. Chua, and S. Kay
Circadian clock mutants in Arabidopsis identified by luciferase imaging
Science, February 24, 1995; 267(5201): 1161 - 1163.
[Abstract] [PDF]

Home page
 ScienceHome page
A. Millar, M Straume, J Chory, N. Chua, and S. Kay
The regulation of circadian period by phototransduction pathways in Arabidopsis
Science, February 24, 1995; 267(5201): 1163 - 1166.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
M. White, M Masuko, L Amet, G Elliott, M Braddock, A. Kingsman, and S. Kingsman
Real-time analysis of the transcriptional regulation of HIV and hCMV promoters in single mammalian cells
J. Cell Sci., January 2, 1995; 108(2): 441 - 455.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
C Bowler, H Yamagata, G Neuhaus, and N H Chua
Phytochrome signal transduction pathways are regulated by reciprocal control mechanisms.
Genes & Dev., September 15, 1994; 8(18): 2188 - 2202.
[Abstract] [PDF]

Home page
 ScienceHome page
W. Jacobs Jr, R. Barletta, R Udani, J Chan, G Kalkut, G Sosne, T Kieser, G. Sarkis, G. Hatfull, and B. Bloom
Rapid assessment of drug susceptibilities of Mycobacterium tuberculosis by means of luciferase reporter phages
Science, May 7, 1993; 260(5109): 819 - 822.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications THE PLANT CELL PLANT PHYSIOLOGY
Copyright © 1992 by the American Society of Plant Biologists