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THE PLANT CELL, Vol 5, Issue 1 109-121, Copyright © 1993 by American Society of Plant Biologists
CA-1, a Novel Phosphoprotein, Interacts with the Promoter of the cab140 Gene in Arabidopsis and Is Undetectable in det1 Mutant Seedlings
L. Sun, R. A. Doxsee, E. Harel and E. M. Tobin
Department of Biology, University of California, Los Angeles, California 90024
We have identified and partially purified a DNA binding protein from
Arabidopsis that interacts specifically with the phytochrome-responsive
promoter of the Arabidopsis cab140 gene. Promoter deletion analyses in
transgenic tobacco showed that, if a region that includes the sequence
interacting with this protein was deleted, both expression and phytochrome
responsiveness were lost. The protein protected a cytosine- and
adenine-rich region from DNase I digestion, and therefore it has been
called CA-1. CA-1 was shown to be a phosphoprotein, and dephosphorylation
changed the migration of the protein-DNA complex in DNA mobility shift
assays. The data suggested that the protein has an apparent molecular
weight of 70,000. The CA-1-protected region of the cab140 promoter included
an ACGT motif that has been found in the target sequences of a number of
bZIP transcription factors, but the binding behavior of CA-1 differed from
those factors. CA-1 binding activity was present in plants grown in either
white light or darkness, and no differences in the binding activity were
detected in the dark-grown plants after short red or white light
treatments. However, the CA-1 binding activity was not detectable in
extracts of seedlings bearing the det1 mutation grown in the dark and given
the same illumination treatments as wild type. In contrast to wild type,
the mutant seedlings express cab RNA at a high level when grown in complete
darkness, and we found no further increase in cab140 mRNA in response to
brief red illumination. The lack of CA-1 activity in the det1 mutant
suggests that it may function as a transcriptional repressor regulating the
expression of the cab140 gene in Arabidopsis.
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