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THE PLANT CELL, Vol 5, Issue 1 39-48, Copyright © 1993 by American Society of Plant Biologists
hy8, a New Class of Arabidopsis Long Hypocotyl Mutants Deficient in Functional Phytochrome A
B. M. Parks and P. H. Quail
University of California-Berkeley/United States Department of Agriculture, Plant Gene Expression Center, 800 Buchanan Street, Albany, California 94710
Emerging evidence suggests that individual members of the phytochrome
family of photoreceptors may regulate discrete facets of plant
photomorphogenesis. We report here the isolation of phytochrome A mutants
of Arabidopsis using a novel screening strategy aimed at detecting
seedlings with long hypocotyls in prolonged far-red light. Complementation
analysis of 10 selected mutant lines showed that each represents an
independent, recessive allele at a new locus, designated hy8. Immunoblot
and spectrophotometric analyses of two of these lines, hy8-1 and hy8-2,
showed that, whereas phytochromes B and C are expressed at wild-type
levels, phytochrome A is undetectable, thus indicating that the long
hypocotyl phenotype displayed by these mutants is caused by phytochrome A
deficiency. A third allele, hy8-3, expresses wild-type levels of spectrally
normal phytochrome A, suggesting a mutation that has resulted in loss of
biological activity in an otherwise photochemically active photoreceptor
molecule. Together with physiological experiments, these data provide
direct evidence that endogenous phytochrome A is responsible for the
"far-red high irradiance response" of etiolated seedlings, but does not
play a major role in mediating responses to prolonged red or white light.
Because the hy8 and the phytochrome B-deficient hy3 mutants exhibit
reciprocal responsivity toward prolonged red and far-red light,
respectively, the evidence indicates that phytochromes A and B have
distinct photosensory roles in regulating seedling development.
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