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THE PLANT CELL, Vol 5, Issue 1 75-86, Copyright © 1993 by American Society of Plant Biologists
Expression of a Self-Incompatibility Gene in a Self-Compatible Line of Brassica oleracea
T. Gaude, A. Friry, P. Heizmann, C. Mariac, M. Rougier, I. Fobis and C. Dumas
Reconnaissance Cellulaire et Amelioration des Plantes, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Universite Claude Bernard-Lyon I, 69 622 Villeurbanne Cedex, France
In cruciferous plants, self-pollination is prevented by the action of genes
situated at the self-incompatibility locus or S-locus. The
self-incompatibility reaction is associated with expression of stigma
glycoproteins encoded by the S-locus glycoprotein (SLG) gene. Only a few
cases of self-compatible plants derived from self-incompatible lines in the
crucifer Brassica have been reported. In these cases, self-compatibility
was generally ascribed to the action of single genes unlinked to the
S-locus. In contrast, we report here a line of Brassica oleracea var
acephala with a self-compatible phenotype linked to the S-locus. By means
of both biochemical and immunochemical analyses, we showed that this
self-compatible (Sc) line nonetheless possesses stigmatic SLGs (SLG-Sc)
that are expressed with a similar spatial and temporal pattern to that
described for the SLGs of self-incompatible Brassica plants. Moreover, the
SLG-Sc products segregate with the self-compatibility phenotype in F2
progeny, suggesting that changes at the S-locus may be responsible for the
occurrence of the self-compatibility character. A cDNA clone encoding the
SLG-Sc product was isolated, and the deduced amino acid sequence showed
this glycoprotein to be highly homologous to the pollen recessive S2 allele
glycoprotein. Hence, self-compatibility in this Brassica Sc line correlates
with the expression of a pollen recessive-like S allele in the stigma.
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