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THE PLANT CELL, Vol 5, Issue 11 1591-1598, Copyright © 1993 by American Society of Plant Biologists
Potato Sucrose Transporter Expression in Minor Veins Indicates a Role in Phloem Loading
J. W. Riesmeier, B. Hirner and W. B. Frommer
Institut fur Genbiologische Forschung, Ihnestrasse 63, 14195 Berlin, Germany
The major transport form of assimilates in most plants is sucrose.
Translocation from the mesophyll into the phloem for long-distance
transport is assumed to be carrier mediated in many species. A sucrose
transporter cDNA was isolated from potato by complementation of a yeast
strain that is unable to grow on sucrose because of the absence of an
endogenous sucrose uptake system and the lack of a secreted invertase. The
deduced amino acid sequence of the potato sucrose transporter gene StSUT1
is highly hydrophobic and is 68% identical to the spinach sucrose
transporter SoSUT1 (pS21). In yeast, the sensitivity of sucrose transport
to protonophores and to an increase in pH is consistent with an active
proton cotransport mechanism. Substrate specificity and inhibition by
protein modifiers are similar to results obtained for sucrose transport
into protoplasts and plasma membrane vesicles and for the spinach
transporter, with the exception of a reduction in maltose affinity. RNA gel
blot analysis shows that the StSUT1 gene is highly expressed in mature
leaves, whereas stem and sink tissues, such as developing leaves, show only
low expression. RNA in situ hybridization studies show that the transporter
gene is expressed specifically in the phloem. Both the properties and the
expression pattern are consistent with a function of the sucrose
transporter protein in phloem loading.
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