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THE PLANT CELL, Vol 5, Issue 12 1749-1759, Copyright © 1993 by American Society of Plant Biologists
Induction of a Highly Specific Antiviral State in Transgenic Plants: Implications for Regulation of Gene Expression and Virus Resistance
J. A. Lindbo, L. Silva-Rosales, W. M. Proebsting and W. G. Dougherty
Department of Microbiology, Oregon State University, Corvallis, Oregon, 97331-3804
Transgenic tobacco plants expressing either a full-length form of the
tobacco etch virus (TEV) coat protein or a form truncated at the N terminus
of the TEV coat protein were initially susceptible to TEV infection, and
typical systemic symptoms developed. However, 3 to 5 weeks after a TEV
infection was established, transgenic plants "recovered" from the TEV
infection, and new stem and leaf tissue emerged symptom and virus free. A
TEV-resistant state was induced in the recovered tissue. The resistance was
virus specific. Recovered plant tissue could not be infected with TEV, but
was susceptible to the closely related virus, potato virus Y. The
resistance phenotype was functional at the single-cell level because
protoplasts from recovered transgenic tissue did not support TEV
replication. Surprisingly, steady state transgene mRNA levels in recovered
tissue were 12-to 22-fold less than transgene mRNA levels in uninoculated
transgenic tissue of the same developmental stage. However, nuclear run-off
studies suggested that transgene transcription rates in recovered and
uninoculated plants were similar. We propose that the resistant state and
reduced steady state levels of transgene transcript accumulation are
mediated at the cellular level by a cytoplasmic activity that targets
specific RNA sequences for inactivation.
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