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THE PLANT CELL, Vol 5, Issue 12 1749-1759, Copyright © 1993 by American Society of Plant Biologists
Induction of a Highly Specific Antiviral State in Transgenic Plants: Implications for Regulation of Gene Expression and Virus Resistance
J. A. Lindbo, L. Silva-Rosales, W. M. Proebsting and W. G. Dougherty
Department of Microbiology, Oregon State University, Corvallis, Oregon, 97331-3804
Transgenic tobacco plants expressing either a full-length form of the
tobacco etch virus (TEV) coat protein or a form truncated at the N terminus
of the TEV coat protein were initially susceptible to TEV infection, and
typical systemic symptoms developed. However, 3 to 5 weeks after a TEV
infection was established, transgenic plants "recovered" from the TEV
infection, and new stem and leaf tissue emerged symptom and virus free. A
TEV-resistant state was induced in the recovered tissue. The resistance was
virus specific. Recovered plant tissue could not be infected with TEV, but
was susceptible to the closely related virus, potato virus Y. The
resistance phenotype was functional at the single-cell level because
protoplasts from recovered transgenic tissue did not support TEV
replication. Surprisingly, steady state transgene mRNA levels in recovered
tissue were 12-to 22-fold less than transgene mRNA levels in uninoculated
transgenic tissue of the same developmental stage. However, nuclear run-off
studies suggested that transgene transcription rates in recovered and
uninoculated plants were similar. We propose that the resistant state and
reduced steady state levels of transgene transcript accumulation are
mediated at the cellular level by a cytoplasmic activity that targets
specific RNA sequences for inactivation.
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|
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|
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|
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|
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|
|
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|
|
|
|
|
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|
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|
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19(3):
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[Full Text]
[PDF]
|
|
|
|
|
|
|
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September 1, 2006;
142(1):
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[Full Text]
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|
|
|
|
|
|
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87(7):
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[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
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September 2, 2005;
309(5740):
1519 - 1524.
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[Full Text]
[PDF]
|
|
|
|
|
|
|
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|
|
|
|
|
|
|
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October 1, 2004;
94(4):
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|
|
|
|
|
|
|
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|
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|
|
|
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85(9):
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|
|
|
|
|
|
|
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|
|
|
|
|
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December 1, 2003;
67(4):
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|
|
|
|
|
|
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|
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|
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|
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|
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|
|
|
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|
|
|
|
|
|
|
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|
|
|
|
|
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|
|
|
|
|
|
|
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|
|
|
|
|
|
|
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|
|
|
|
|
|
|
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[Full Text]
[PDF]
|
|
|
|
|
|
|
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February 15, 2002;
76(4):
1922 - 1931.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Tenllado and J. R. Diaz-Ruiz
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J. Virol.,
December 15, 2001;
75(24):
12288 - 12297.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-H. Chiang, J.-J. Wang, F.-J. Jan, S.-D. Yeh, and D. Gonsalves
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J. Gen. Virol.,
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82(11):
2827 - 2836.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. I. Savenkov and J. P. T. Valkonen
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J. Gen. Virol.,
September 1, 2001;
82(9):
2275 - 2278.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. A. Miller, P. M. Waterhouse, J. W. S. Brown, and K. S. Browning
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August 1, 2001;
13(8):
1710 - 1717.
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Vance and H. Vaucheret
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Science,
June 22, 2001;
292(5525):
2277 - 2280.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. V. Sidorenko and T. Peterson
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[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Vaucheret, C. Beclin, and M. Fagard
Post-transcriptional gene silencing in plants
J. Cell Sci.,
January 9, 2001;
114(17):
3083 - 3091.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F.-J. Jan, S.-Z. Pang, D. M. Tricoli, and D. Gonsalves
Evidence that resistance in squash mosaic comovirus coat protein-transgenic plants is affected by plant developmental stage and enhanced by combination of transgenes from different lines
J. Gen. Virol.,
September 1, 2000;
81(9):
2299 - 2306.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H.-H. Yeh, T. Tian, L. Rubio, B. Crawford, and B. W. Falk
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J. Virol.,
July 1, 2000;
74(13):
5762 - 5768.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
W. M. Wintermantel and M. Zaitlin
Transgene translatability increases effectiveness of replicase-mediated resistance to Cucumber mosaic virus
J. Gen. Virol.,
March 1, 2000;
81(3):
587 - 595.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Dalmay, A. Hamilton, E. Mueller, and D. C. Baulcombe
Potato Virus X Amplicons in Arabidopsis Mediate Genetic and Epigenetic Gene Silencing
PLANT CELL,
March 1, 2000;
12(3):
369 - 380.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F.-J. Jan, C. Fagoaga, S.-Z. Pang, and D. Gonsalves
A minimum length of N gene sequence in transgenic plants is required for RNA-mediated tospovirus resistance
J. Gen. Virol.,
January 1, 2000;
81(1):
235 - 242.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. Cogoni and G. Macino
Posttranscriptional Gene Silencing in Neurospora by a RecQ DNA Helicase
Science,
December 17, 1999;
286(5448):
2342 - 2344.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
L. Jones, A. J. Hamilton, O. Voinnet, C. L. Thomas, A. J. Maule, and D. C. Baulcombe
RNA-DNA Interactions and DNA Methylation in Post-Transcriptional Gene Silencing
PLANT CELL,
December 1, 1999;
11(12):
2291 - 2302.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. J. Hamilton and D. C. Baulcombe
A Species of Small Antisense RNA in Posttranscriptional Gene Silencing in Plants
Science,
October 29, 1999;
286(5441):
950 - 952.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. P. Wolffe and M. A. Matzke
Epigenetics: Regulation Through Repression
Science,
October 15, 1999;
286(5439):
481 - 486.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. G. Ratcliff, S. A. MacFarlane, and D. C. Baulcombe
Gene Silencing without DNA: RNA-Mediated Cross-Protection between Viruses
PLANT CELL,
July 1, 1999;
11(7):
1207 - 1216.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
I. L. Ingelbrecht, J. E. Irvine, and T. E. Mirkov
Posttranscriptional Gene Silencing in Transgenic Sugarcane. Dissection of Homology-Dependent Virus Resistance in a Monocot That Has a Complex Polyploid Genome
Plant Physiology,
April 1, 1999;
119(4):
1187 - 1198.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Holtorf, H. Schob, C. Kunz, R. Waldvogel, and F. Meins , Jr.
Stochastic and Nonstochastic Post-Transcriptional Silencing of Chitinase and ß-1,3-Glucanase Genes Involves Increased RNA Turnover—Possible Role for Ribosome-Independent RNA Degradation
PLANT CELL,
March 1, 1999;
11(3):
471 - 484.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R Ruiz-Medrano, B Xoconostle-Cazares, and W. Lucas
Phloem long-distance transport of CmNACP mRNA: implications for supracellular regulation in plants
Development,
January 10, 1999;
126(20):
4405 - 4419.
[Abstract]
[PDF]
|
|
|
|
|
|
|
W. Schiebel, T. Pélissier, L. Riedel, S. Thalmeir, R. Schiebel, D. Kempe, F. Lottspeich, H. L. Sänger, and M. Wassenegger
Isolation of an RNA-Directed RNA Polymerase –Specific cDNA Clone from Tomato
PLANT CELL,
December 1, 1998;
10(12):
2087 - 2102.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. Bruening
Plant gene silencing regularized
PNAS,
November 10, 1998;
95(23):
13349 - 13351.
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Stam, A. Viterbo, J. N. M. Mol, and J. M. Kooter
Position-Dependent Methylation and Transcriptional Silencing of Transgenes in Inverted T-DNA Repeats: Implications for Posttranscriptional Silencing of Homologous Host Genes in Plants
Mol. Cell. Biol.,
November 1, 1998;
18(11):
6165 - 6177.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. Anandalakshmi, G. J. Pruss, X. Ge, R. Marathe, A. C. Mallory, T. H. Smith, and V. B. Vance
A viral suppressor of gene silencing in plants
PNAS,
October 27, 1998;
95(22):
13079 - 13084.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Elmayan, S. Balzergue, F. Béon, V. Bourdon, J. Daubremet, Y. Guénet, P. Mourrain, J.-C. Palauqui, S. Vernhettes, T. Vialle, et al.
Arabidopsis Mutants Impaired in Cosuppression
PLANT CELL,
October 1, 1998;
10(10):
1747 - 1758.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J.-C. Palauqui and H. Vaucheret
Transgenes are dispensable for the RNA degradation step of cosuppression
PNAS,
August 4, 1998;
95(16):
9675 - 9680.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. T. Ruiz, O. Voinnet, and D. C. Baulcombe
Initiation and Maintenance of Virus-Induced Gene Silencing
PLANT CELL,
June 1, 1998;
10(6):
937 - 946.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. Cogoni and G. Macino
Isolation of quelling-defective (qde) mutants impaired in posttranscriptional transgene-induced gene silencing in Neurospora crassa
PNAS,
September 16, 1997;
94(19):
10233 - 10238.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-Z. Pang, F.-J. Jan, and D. Gonsalves
Nontarget DNA sequences reduce the transgene length necessary for RNA-mediated tospovirus resistance in transgenic plants
PNAS,
July 22, 1997;
94(15):
8261 - 8266.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Jorgensen
Cosuppression, Flower Color Patterns, and Metastable Gene Expression States
Science,
May 5, 1995;
268(5211):
686 - 691.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. Fagard, S. Boutet, J.-B. Morel, C. Bellini, and H. Vaucheret
AGO1, QDE-2, and RDE-1 are related proteins required for post-transcriptional gene silencing in plants, quelling in fungi, and RNA interference in animals
PNAS,
October 10, 2000;
97(21):
11650 - 11654.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
