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THE PLANT CELL, Vol 5, Issue 2 159-169, Copyright © 1993 by American Society of Plant Biologists
Regulation of Pathogenesis-Related Protein-1a Gene Expression in Tobacco
S. Uknes, S. Dincher, L. Friedrich, D. Negrotto, S. Williams, H. Thompson-Taylor, S. Potter, E. Ward and J. Ryals
Agricultural Biotechnology Research Unit, CIBA-GEIGY Corporation, 3054 Cornwallis Road, Research Triangle Park, North Carolina 27709
Pathogenesis-related protein-1a (PR-1a) is a protein of unknown function
that is strongly induced during the onset of systemic acquired resistance
(SAR) in tobacco. The expression of PR-1a is under complex regulation that
is controlled at least partially by the rate of transcription. In this
study, we demonstrated that 661 bp of 5[prime] flanking DNA was sufficient
to impart tobacco mosaic virus and salicylic acid inducibility to a
reporter gene. The PR-1a promoter did not respond significantly to
treatments with either auxin or cytokinin. Experiments with the protein
synthesis inhibitor cycloheximide indicated that protein synthesis is
required for salicylate-dependent mRNA accumulation. At flowering, the
PR-1a gene was expressed primarily in the mesophyll and epidermal tissues
of the leaf blade and the sepals of the flower. Several artifacts, most
importantly ectopic expression in pollen, were associated with the use of
the [beta]-glucuronidase reporter gene.
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