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THE PLANT CELL, Vol 5, Issue 2 181-189, Copyright © 1993 by American Society of Plant Biologists
Uncoupling Auxin and Ethylene Effects in Transgenic Tobacco and Arabidopsis Plants
C. P. Romano, M. L. Cooper and H. J. Klee
Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, Missouri 63198
Overproduction of auxin in transgenic plants also results in the
overproduction of ethylene. Plants overproducing both auxin and ethylene
display inhibition of stem elongation and growth, increased apical
dominance, and leaf epinasty. To determine the relative roles of auxin and
ethylene in these processes, transgenic tobacco and Arabidopsis plants
expressing the auxin-overproducing tryptophan monooxygenase transgene were
crossed to plants expressing an ethylene synthesis-inhibiting
1-aminocyclopropane-1-carboxylate deaminase transgene. Tobacco and
Arabidopsis plants with elevated auxin and normal levels of ethylene were
obtained by this strategy. Transgenic auxin-overproducing Arabidopsis
plants were also crossed with the ethylene-insensitive ein1 and ein2
mutants. Analysis of these plants indicates that apical dominance and leaf
epinasty are primarily controlled by auxin rather than ethylene. However,
ethylene is partially responsible for the inhibition of stem elongation
observed in auxin-overproducing tobacco. Finally, these data show that
auxin overproduction can be effectively uncoupled from ethylene
overproduction in transgenic plants to enable direct manipulation of plant
morphology for agronomic and horticultural purposes.
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