|
THE PLANT CELL, Vol 5, Issue 3 253-261, Copyright © 1993 by American Society of Plant Biologists
Genetic Ablation of Floral Cells in Arabidopsis
M. K. Thorsness, M. K. Kandasamy, M. E. Nasrallah and J. B. Nasrallah
Section of Plant Biology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853
A chimeric toxic gene consisting of the diphtheria toxin A chain gene fused
to a promoter previously shown to direct pistil- and anther-specific
expression was used to genetically target cell killing in transgenic
Arabidopsis. Flowers of Arabidopsis transformants that carried the toxic
gene fusion had distinct structural defects. The papillar cells at the
stigma surface were stunted and were biosynthetically inactive. Anther
development was also impaired by toxic gene expression, leading to
abnormalities in anther dehiscence, pollen morphology, and pollen
germination. The combined defects of pistil and anther rendered
transformants that carried the toxic gene fusion self-sterile. However, the
transformants were cross-fertile with untransformed plants: the viable
pollen of ablated plants was rescued by wild-type stigmas, and, strikingly,
the ablated papillar cells allowed the growth of wild-type pollen.
This article has been cited by other articles:
|
|
|
|
 
A. C. Frank and M. A. Johnson
Expressing the Diphtheria Toxin A Subunit from the HAP2(GCS1) Promoter Blocks Sperm Maturation and Produces Single Sperm-Like Cells Capable of Fertilization
Plant Physiology,
November 1, 2009;
151(3):
1390 - 1400.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. L. Schilmiller, A. J.K. Koo, and G. A. Howe
Functional Diversification of Acyl-Coenzyme A Oxidases in Jasmonic Acid Biosynthesis and Action
Plant Physiology,
February 1, 2007;
143(2):
812 - 824.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Yang, R. A. Jefferson, E. Huttner, J. M. Moore, W. B. Gagliano, and U. Grossniklaus
An Egg Apparatus-Specific Enhancer of Arabidopsis, Identified by Enhancer Detection
Plant Physiology,
November 1, 2005;
139(3):
1421 - 1432.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. L. Engel, R. Holmes-Davis, and S. McCormick
Green Sperm. Identification of Male Gamete Promoters in Arabidopsis
Plant Physiology,
August 1, 2005;
138(4):
2124 - 2133.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-W. Tung, K. G. Dwyer, M. E. Nasrallah, and J. B. Nasrallah
Genome-Wide Identification of Genes Expressed in Arabidopsis Pistils Specifically along the Path of Pollen Tube Growth
Plant Physiology,
June 1, 2005;
138(2):
977 - 989.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. F. Edlund, R. Swanson, and D. Preuss
Pollen and Stigma Structure and Function: The Role of Diversity in Pollination
PLANT CELL,
June 1, 2004;
16(suppl_1):
S84 - S97.
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-H. Kang, J. S. Busse, and S. Y. Bednarek
Members of the Arabidopsis Dynamin-Like Gene Family, ADL1, Are Essential for Plant Cytokinesis and Polarized Cell Growth
PLANT CELL,
April 1, 2003;
15(4):
899 - 913.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Mouline, A.-A. Very, F. Gaymard, J. Boucherez, G. Pilot, M. Devic, D. Bouchez, J.-B. Thibaud, and H. Sentenac
Pollen tube development and competitive ability are impaired by disruption of a Shaker K+ channel in Arabidopsis
Genes & Dev.,
February 1, 2002;
16(3):
339 - 350.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hill, C. Day, S. Zondlo, A. Thackeray, and V. Irish
Discrete spatial and temporal cis-acting elements regulate transcription of the Arabidopsis floral homeotic gene APETALA3
Development,
January 5, 1998;
125(9):
1711 - 1721.
[Abstract]
[PDF]
|
|
|
|
|
|
|
C. Day, B. Galgoci, and V. Irish
Genetic ablation of petal and stamen primordia to elucidate cell interactions during floral development
Development,
January 9, 1995;
121(9):
2887 - 2895.
[Abstract]
[PDF]
|
|
|
|
|
