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THE PLANT CELL, Vol 5, Issue 6 657-666, Copyright © 1993 by American Society of Plant Biologists
An Alternative Transcript of the S Locus Glycoprotein Gene in a Class II Pollen-Recessive Self-Incompatibility Haplotype of Brassica oleracea Encodes a Membrane-Anchored Protein
T. Tantikanjana, M. E. Nasrallah, J. C. Stein, C. H. Chen and J. B. Nasrallah
Section of Plant Biology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853
Recent reports have shown that SLG, one of two genes linked to the S locus
of Brassica, encodes a secreted glycoprotein. We have used RNA gel blot
analysis, genomic and cDNA clone analysis, expression in transgenic plants,
and immunodetection to characterize SLG2, the SLG gene derived from the S2
haplotype. This haplotype belongs to the class II group of S haplotypes
that exhibit a weak incompatibility phenotype and are pollen recessive. We
showed that SLG2 produces two transcript forms: the expected 1.6-kb
transcript that predicts a secreted glycoprotein and an alternative 1.8-kb
transcript that predicts a membrane-anchored protein. Stigmas of the S2
haplotype and pistils of transgenic tobacco plants transformed with the
SLG2 gene produce a membrane-associated 62-kD protein as well as soluble
57- and 58-kD glycoforms. Because of the sequence similarity between SLG2
and the extracellular domain of the S Locus Receptor Kinase (SRK2) gene,
the membrane-anchored form of SLG2 may be viewed as a naturally occurring
truncated form of the receptor that lacks the kinase catalytic domain. The
occurrence of this protein has potential implications for the activity of
the full-length receptor. Furthermore, the underlying structure of the SLG2
gene suggests the evolution of SLG from an ancestral SRK-like gene.
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