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THE PLANT CELL, Vol 5, Issue 7 739-746, Copyright © 1993 by American Society of Plant Biologists
In Vitro Fertilization with Isolated, Single Gametes Results in Zygotic Embryogenesis and Fertile Maize Plants
E. Kranz and H. Lorz
Institut fur Allgemeine Botanik, Angewandte Molekularbiologie der Pflanzen II, Universitat Hamburg, Ohnhorststrasse 18, 22609 Hamburg, Germany
We demonstrate here the possibility of regenerating phenotypically normal,
fertile maize plants via in vitro fertilization of isolated, single sperm
and egg cells mediated by electrofusion. The technique leads to the highly
efficient formation of polar zygotes, globular structures, proembryos, and
transition-phase embryos and to the formation of plants from individually
cultured fusion products. Regeneration of plants occurs via embryogenesis
and occasionally by polyembryony and organogenesis. Flowering plants can be
obtained within 100 days of gamete fusion. Regenerated plants were studied
by karyological and morphological analyses, and the segregation of kernel
color was determined. The hybrid nature of the plants was confirmed.
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