THE PLANT CELL, Vol 5, Issue 9 1011-1027, Copyright © 1993 by American Society of Plant Biologists

RESEARCH ARTICLES

Suppressors of trp1 Fluorescence Identify a New Arabidopsis Gene, TRP4, Encoding the Anthranilate Synthase [beta] Subunit

K. K. Niyogi, R. L. Last, G. R. Fink and B. Keith
Department of Biology and Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, Massachusetts 02142

Suppressors of the blue fluorescence phenotype of the Arabidopsis trp1-100 mutant can be used to identify mutations in genes involved in plant tryptophan biosynthesis. Two recessive suppressor mutations define a new gene, TRP4. The trp4 mutant and the trp1-100 mutant are morphologically normal and grow without tryptophan, whereas the trp4; trp1-100 double mutant requires tryptophan for growth. The trp4; trp1-100 double mutant does not segregate at expected frequencies in genetic crosses because of a female-specific defect in transmission of the double mutant genotype, suggesting a role for the tryptophan pathway in female gametophyte development. Genetic and biochemical evidence shows that trp4 mutants are defective in a gene encoding the [beta] subunit of anthranilate synthase (AS). Arabidopsis AS [beta] subunit genes were isolated by complementation of an Escherichia coli anthranilate synthase mutation. The trp4 mutation co-segregates with one of the genes, ASB1, located on chromosome 1. Sequence analysis of the ASB1 gene from trp4-1 and trp4-2 plants revealed different single base pair substitutions relative to the wild type. Anthranilate synthase [alpha] and [beta] subunit genes are regulated coordinately in response to bacterial pathogen infiltration.

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