|
THE PLANT CELL, Vol 5, Issue 9 1125-1138, Copyright © 1993 by American Society of Plant Biologists
Jordan, an Active Volvox Transposable Element Similar to Higher Plant Transposons
S. M. Miller, R. Schmitt and D. L. Kirk
Department of Biology, Washington University, St. Louis, Missouri 63130
We have isolated a 1595-bp transposable element from the multicellular
green alga Volvox carteri following its insertion into the nitrate
reductase (nitA) locus. This element, which we have named Jordan, has short
(12-bp) terminal inverted repeats and creates a 3-bp target site
duplication, like some higher plant transposons of the classic type.
Contained within the first 200 bp of one end of the element are 55-bp
inverted repeats, one of which begins with the terminal inverted repeat.
Revertants of the transposon insertion into the nitA locus were obtained at
a rate of ~10-4 per Volvox embryo per generation. In each revertant
examined, all transposon sequences were completely excised, but footprints
containing both sets of duplicated bases, in addition to three to nine
extra bases, were left behind. Jordan contains no significant open reading
frames and so appears to be nonautonomous. DNA gel blot analysis indicates
that Jordan is a member of a large family of homologous elements in the
Volvox genome. We have isolated and characterized several of these homologs
and found that they contain termini very similar to those of Jordan.
Efforts to utilize Jordan and its homologs as tools to tag and clone
developmentally interesting genes of Volvox are discussed.
This article has been cited by other articles:
|
|
|
|
 
T. Hamaji, P. J. Ferris, A. W. Coleman, S. Waffenschmidt, F. Takahashi, I. Nishii, and H. Nozaki
Identification of the Minus-Dominance Gene Ortholog in the Mating-Type Locus of Gonium pectorale
Genetics,
January 1, 2008;
178(1):
283 - 294.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K.-S. Kim, S. Kustu, and W. Inwood
Natural History of Transposition in the Green Alga Chlamydomonas reinhardtii: Use of the AMT4 Locus as an Experimental System
Genetics,
August 1, 2006;
173(4):
2005 - 2019.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Nedelcu and R. E. Michod
The Evolutionary Origin of an Altruistic Gene
Mol. Biol. Evol.,
August 1, 2006;
23(8):
1460 - 1464.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. L. Kirk
Seeking the Ultimate and Proximate Causes of Volvox Multicellularity and Cellular Differentiation
Integr. Comp. Biol.,
April 1, 2003;
43(2):
247 - 253.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Duncan, K. Bouckaert, F. Yeh, and D. L. Kirk
kangaroo, a Mobile Element From Volvox carteri, Is a Member of a Newly Recognized Third Class of Retrotransposons
Genetics,
December 1, 2002;
162(4):
1617 - 1630.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Cove
The generation and modification of cell polarity
J. Exp. Bot.,
May 1, 2000;
51(346):
831 - 838.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I Nishii and S Ogihara
Actomyosin contraction of the posterior hemisphere is required for inversion of the Volvox embryo
Development,
January 5, 1999;
126(10):
2117 - 2127.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. Kirk, K Stark, S. Miller, W Muller, B. Taillon, H Gruber, R Schmitt, and D. Kirk
regA, a Volvox gene that plays a central role in germ-soma differentiation, encodes a novel regulatory protein
Development,
January 2, 1999;
126(4):
639 - 647.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. Miller and D. Kirk
glsA, a Volvox gene required for asymmetric division and germ cell specification, encodes a chaperone-like protein
Development,
January 2, 1999;
126(4):
649 - 658.
[Abstract]
[PDF]
|
|
|
|
|
