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THE PLANT CELL, Vol 6, Issue 12 1815-1828, Copyright © 1994 by American Society of Plant Biologists

RESEARCH ARTICLES

Pollen Tube Growth Is Coupled to the Extracellular Calcium Ion Flux and the Intracellular Calcium Gradient: Effect of BAPTA-Type Buffers and Hypertonic Media

E. S. Pierson, D. D. Miller, D. A. Callaham, A. M. Shipley, B. A. Rivers, M. Cresti and P. K. Hepler
Department of Biology, Morrill Science Center III, University of Massachusetts, Amherst, Massachusetts 01003

Lily pollen tubes possess a steep, tip-focused intracellular Ca2+ gradient and a tip-directed extracellular Ca2+ influx. Ratiometric ion imaging revealed that the gradient extends from above 3.0 [mu]M at the apex to ~0.2 [mu]M within 20 [mu]m from the tip, while application of the Ca2+-specific vibrating electrode indicated that the extracellular influx measured between 1.4 and 14 pmol cm-2 sec-1. We examined the relationship between these phenomena and their role in tube growth by using different 1,2-bis(o-aminophenoxy)ethane N,N,N[prime],N[prime]-tetraacetic acid (BAPTA)-type buffers and hypertonic media. Injection of active BAPTA-type buffers or application of elevated levels of sucrose reversibly inhibited growth, destroyed tip zonation of organelles, and modified normal patterns of cytoplasmic streaming. Simultaneously, these treatments dissipated both the intracellular tip-focused gradient and the extracellular Ca2+ flux. Of the BAPTA-type buffers, 5,5[prime]-dibromo-BAPTA (dissociation constant [Kd] is 1.5 [mu]M) and 4,4[prime]-difluoro-BAPTA (Kd of 1.7 [mu]M) exhibited greater activity than those buffers with either a higher affinity (5,5[prime]-dimethyl-BAPTA, Kd of 0.15 [mu]M; BAPTA, Kd of 0.21 [mu]M; 5,5[prime]- difluoro-BAPTA, Kd of 0.25 [mu]M) or lower affinity (5-methyl, 5[prime]-nitro-BAPTA, Kd of 22 [mu]M) for Ca2+. Our findings provide evidence that growing pollen tubes have open Ca2+ channels in their tip and that these channels become inactivated in nongrowing tubes. The studies with elevated sucrose support the view that stretching of the apical plasma membrane contributes to the maintenance of the Ca2+ signal.


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