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THE PLANT CELL, Vol 6, Issue 3 351-360, Copyright © 1994 by American Society of Plant Biologists
The Maize Abscisic Acid-Responsive Protein Rab17 Is Located in the Nucleus and Interacts with Nuclear Localization Signals
A. Goday, A. B. Jensen, F. A. Culianez-Macia, M. M. Alba, M. Figueras, J. Serratosa, M. Torrent and M. Pages
Departament de Genetica Molecular, Centre d'Investigacio i Desenvolupament,Consejo Superior de Investigaciones Cientificas, Jorge Girona 18-26, 08034 Barcelona, Spain
The maize abscisic acid (ABA)-responsive rab17 mRNA and Rab17 protein
distribution in maize embryo tissues was investigated by in situ
hybridization and immunocytochemistry. rab17 mRNA and Rab17 protein were
found in all cells of embryo tissues. Synthesis of rab17 mRNA occurred
initially in the embryo axis. As maturation progressed, rab17 mRNA was
detectable in the scutellum and accumulated in axis cells and provascular
tissues. However, the response to exogenous ABA differed in various embryo
cell types. The Rab17 protein was located in the nucleus and in the
cytoplasm, and qualitative differences in the phosphorylation states of the
protein were found between the two subcellular compartments. Based on the
similar domain arrangements of Rab17 and a nuclear localization signal
(NLS) binding phosphoprotein, Nopp140, interaction of Rab17 with NLS
peptides was studied. We found specific binding of Rab17 to the wild-type
NLS of the SV40 T antigen but not to an import incompetent mutant peptide.
Moreover, binding of the NLS peptide to Rab17 was found to be dependent
upon phosphorylation. These results suggest that Rab17 may play a role in
nuclear protein transport.
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