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THE PLANT CELL, Vol 6, Issue 3 449-460, Copyright © 1994 by American Society of Plant Biologists
Dominant Negative Suppression of Arabidopsis Photoresponses by Mutant Phytochrome A Sequences Identifies Spatially Discrete Regulatory Domains in the Photoreceptor
M. Boylan, N. Douglas and P. H. Quail
University of California-Berkeley/U. S. Department of Agriculture, Plant Gene Expression Center, 800 Buchanan Street, Albany, California 94710
We used the exaggerated short hypocotyl phenotype induced by oat
phytochrome A overexpression in transgenic Arabidopsis to monitor the
biological activity of mutant phytochrome A derivatives. Three different
mutations, which were generated by removing 52 amino acids from the N
terminus ([delta]N52), the entire C-terminal domain ([delta]C617), or amino
acids 617-686 ([delta]617-686) of the oat molecule, each caused striking
dominant negative interference with the ability of endogenous Arabidopsis
phytochrome A to inhibit hypocotyl growth in continuous far-red light
("far-red high irradiance response" conditions). By contrast, in continuous
white or red light, [delta]N52 was as active as the unmutagenized oat
phytochrome A protein in suppressing hypocotyl elongation, while
[delta]C617 and [delta]617-686 continued to exhibit dominant negative
behavior under these conditions. These data suggest that at least three
spatially discrete molecular domains coordinate the photoregulatory
activities of phytochrome A in Arabidopsis seedlings. The first is the
chromophore-bearing N-terminal domain between residues 53 and 616 that is
apparently sufficient for the light-induced initiation but not the
completion of productive interactions with transduction chain components.
The second is the C-terminal domain between residues 617 and 1129 that is
apparently necessary for completion of productive interactions under all
irradiation conditions. The third is the N-terminal 52 amino acids that are
apparently necessary for completion of productive interactions only under
far-red high irradiance conditions and are completely dispensable under
white and red light regimes.
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