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THE PLANT CELL, Vol 6, Issue 5 695-708, Copyright © 1994 by American Society of Plant Biologists
Plant Homeodomain Protein Involved in Transcriptional Regulation of a Pathogen Defense-Related Gene
U. Korfhage, G. F. Trezzini, I. Meier, K. Hahlbrock and I. E. Somssich
Max-Planck-Institut fur Zuchtungsforschung, Abteilung Biochemie, D-50829 Koln, Germany
Transcription of the parsley pr2 gene, encoding pathogenesis-related
protein 2 (PR2), is rapidly stimulated by fungal or bacterial elicitors.
Previous work has revealed a 125-bp region within the pr2 promoter; this
region encompasses all important cis-regulatory elements required for
fungal elicitor-mediated expression. We now report the identification of a
functionally relevant 11-bp DNA motif (CTAATTGTTTA) contained within this
region; it specifically binds to factors present in both parsley and
Arabidopsis nuclear protein extracts. From both plant species, full-length
cDNA clones were isolated that encode proteins with high affinity
for this DNA motif. The proteins from both species contain stretches of 61
amino acids that are characteristic of homeodomain (HD) proteins. Binding
studies and use of a polyclonal antiserum raised against a fusion
polypeptide of glutathione S-transferase with the HD portion of the parsley
protein indicated that the 11-bp DNA motif is a potential in vivo target
site and that the HD protein is contained within the observed complex
formed between the DNA motif and nuclear protein extracts. Transient
expression studies using the authentic and a mutated target site suggested
a functional role of the HD-DNA interaction in the regulation of the pr2
gene expression.
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