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THE PLANT CELL, Vol 6, Issue 5 737-749, Copyright © 1994 by American Society of Plant Biologists
Phosphate Modulates Transcription of Soybean VspB and Other Sugar-Inducible Genes
A. Sadka, D. B. DeWald, G. D. May, W. D. Park and J. E. Mullet
Department of Biochemistry and Biophysics, Texas A & M University, College Station, Texas 77843-2128
The soybean vegetative storage protein genes VspA and VspB encode vacuolar
glycoprotein acid phosphatases. Transcription of the Vsp is synergistically
activated by jasmonic acid or methyl jasmonate (MeJA) and soluble sugars.
The action of these modulators is mediated by two different DNA domains in
the VspB promoter. In this study, we present new data regarding VspB
regulation by sucrose and inorganic phosphate, which suggest a common
mechanism of transcriptional control for Vsp and other sugar-inducible
genes. We found that the sugar-mediated activation of VspB expression was
inhibited by phosphate. Deletion analysis and transient assays in tobacco
protoplasts identified a 130-bp DNA domain in the VspB promoter that
mediates both sucrose induction and phosphate inhibition. Transcription
mediated by this DNA domain was induced by phosphate elimination from the
protoplast incubation medium, even in the absence of sucrose. The effect of
sucrose and phosphate on VspB expression was studied in vivo in several
ways. Depletion of phosphate from soybean cell cultures by the addition of
mannose stimulated VspB expression, even in the absence of sucrose or MeJA.
In illuminated soybean leaves treated with MeJA, inhibition of
photosynthetic electron transport by DCMU decreased VspB expression. In
contrast, VspB expression in soybean leaves stimulated by phosphate
depletion was not influenced by DCMU. Moreover, sucrose-stimulated
expression of the sugar-responsive genes lipoxygenase A and chalcone
synthase of soybean and proteinase inhibitor II and class I patatin of
potato was inhibited by phosphate. Like VspB, these genes were stimulated
by phosphate depletion in the absence of exogenous sucrose. We propose that
sugar-responsive genes are activated, in part, by accumulation of
sugar-phosphates and concomitant reduction of cellular phosphate levels.
These data may help explain recruitment of the Vsp, which encode acid
phosphatases, as vegetative storage proteins.
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