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THE PLANT CELL, Vol 6, Issue 5 761-772, Copyright © 1994 by American Society of Plant Biologists
Carbon Catabolite Repression Regulates Glyoxylate Cycle Gene Expression in Cucumber
I. A. Graham, K. J. Denby and C. J. Leaver
Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, United Kingdom
We have previously proposed that metabolic status is important in the
regulation of cucumber malate synthase (MS) and isocitrate lyase (ICL) gene
expression during plant development. In this article, we used a cell
culture system to demonstrate that intracellular metabolic status does
influence expression of both of these genes. Starvation of cucumber cell
cultures resulted in the coordinate induction of the expression of MS and
ICL genes, and this effect was reversed when sucrose was returned to the
culture media. The induction of gene expression was closely correlated with
a drop in intracellular sucrose, glucose, and fructose below threshold
concentrations, but it was not correlated with a decrease in respiration
rate. Glucose, fructose, or raffinose in the culture media also resulted in
repression of MS and ICL. Both 2-deoxyglucose and mannose, which are
phosphorylated by hexokinase but not further metabolized, specifically
repressed MS and ICL gene expression relative to a third glyoxylate cycle
gene, malate dehydrogenase. However, the addition of 3-methylglucose, an
analog of glucose that is not phosphorylated, did not result in repression
of either MS or ICL. It is proposed that the signal giving rise to a change
in gene expression originates from the intracellular concentration of
hexose sugars or the flux of hexose sugars into glycolysis.
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