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THE PLANT CELL, Vol 6, Issue 7 935-945, Copyright © 1994 by American Society of Plant Biologists

RESEARCH ARTICLES

Cutinase Gene Disruption in Fusarium solani f sp pisi Decreases Its Virulence on Pea

L. M. Rogers, M. A. Flaishman and P. E. Kolattukudy
Ohio State Biotechnology Center, Rightmire Hall, 1060 Carmack Road, The Ohio State University, Columbus, Ohio 43210-1002

Fusarium solani f sp pisi (Nectria haematococca) isolate 77-2-3 with one cutinase gene produced 10 to 20% of the cutinase produced by isolate T-8 that has multiple cutinase genes, whereas cutinase gene-disrupted mutant 77-102 of isolate 77-2-3 did not produce cutinase. On the surface of pea stem segments, lesion formation was most frequent and most severe with T-8, less frequent and less severe with 77-2-3, and much less frequent and much milder with the gene-disrupted mutant. Microscopic examination of the lesions caused by the mutant strongly suggested that it penetrated the host mostly via the stomata. In seedling assays, 77-2-3 caused severe lesions on every seedling and stunted growth, whereas the mutant showed very mild lesions on one-third of the seedlings with no stunting. Thus, cutinase gene disruption resulted in a significant decrease in the pathogenicity of F. s. pisi on pea.


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Copyright © 1994 by the American Society of Plant Biologists