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THE PLANT CELL, Vol 6, Issue 7 947-958, Copyright © 1994 by American Society of Plant Biologists
A New Family of Zinc Finger Proteins in Petunia: Structure, DNA Sequence Recognition, and Floral Organ-Specific Expression
H. Takatsuji, N. Nakamura and Y. Katsumoto
Laboratory of Developmental Biology, National Institute of Agrobiological Resources, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305 Japan
We have previously cloned a gene for a zinc finger protein (EPF1) that is
expressed specifically in petals and interacts with the promoter region of
the 5-enolpyruvylshikimate-3-phosphate synthase gene in petunia. In an
attempt to isolate genes encoding additional factors that interact with
this promoter, we cloned four novel genes encoding zinc finger proteins
(EPF2-5a, EPF2-5b, EPF2-4, and EPF2-7). Sequence analyses revealed that
overall similarity between the EPF1 and the EPF2 protein family, except in
the zinc finger motifs and the basic amino acid cluster, was very low,
suggesting that the two groups belong to different subfamilies. DNA binding
specificities of EPF1, EPF2-5, and EPF2-4 were very similar, as expected
from the conserved zinc finger motifs. However, EPF2-7 showed no binding to
the probes tested in spite of having the conserved motifs. DNA binding
studies using a series of spacing mutant probes suggested a binding
mechanism in which the EPF proteins recognize spacings in target DNA. RNA
gel blot analyses and histochemical analyses with a promoter and
[beta]-glucuronidase fusion revealed that expression of the EPF2-5 gene
(EPF2-5) was petal and stamen specific. Expression of the EPF2-7 gene
(EPF2-7) was sepal and petal specific and localized in vascular tissues.
The preferential expression in two adjacent floral organs raises the
possibility that these genes are downstream transcription factors of floral
homeotic genes.
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