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THE PLANT CELL, Vol 6, Issue 8 1087-1098, Copyright © 1994 by American Society of Plant Biologists
A Dominant Negative Mutant of PG13 Suppresses Transcription from a Cauliflower Mosaic Virus 35S Truncated Promoter in Transgenic Tobacco Plants
M. Rieping, M. Fritz, S. Prat and C. Gatz
Universitat Bielefeld, Lehrstuhl fur Genetik, Fakultat fur Biologie, 33501 Bielefeld, Germany
TGA1a and PG13 constitute a family of tobacco basic leucine zipper (bZIP)
proteins that bind to activating sequence-1 (as-1), which is one of the
multiple regulatory cis elements of the cauliflower mosaic virus (CaMV) 35S
promoter. After truncation of the CaMV 35S promoter down to position -90
(CaMV 35S [-90] promoter), transcription stringently depends on the
presence of as-1, which is recognized by nuclear DNA binding proteins
called ASF-1. The role of the TGA1a/PG13 bZIP family in the formation of
ASF-1 and in transcriptional activation of the CaMV 35S (-90) promoter has
not yet been demonstrated in vivo. We constructed transgenic tobacco plants
expressing a mutant of potato PG13, which lacks its wild-type DNA binding
domain. This mutant acts as a trans-dominant inhibitor of ASF-1 formation
and of expression from the CaMV 35S (-90) promoter, showing that PG13 can
specifically interact with proteins necessary for these processes. Although
we did not observe any other obvious phenotypic changes, these transgenic
plants are a potentially valuable tool in identifying whether TGA1a and
PG13 are involved in controlling promoters encoded in the plant genome.
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