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THE PLANT CELL, Vol 6, Issue 9 1301-1310, Copyright © 1994 by American Society of Plant Biologists
Oxidative Signals in Tobacco Increase Cytosolic Calcium
A. H. Price, A. Taylor, S. J. Ripley, A. Griffiths, A. J. Trewavas and M. R. Knight
School of Biological Sciences, University of Wales-Bangor, Bangor, Gwynedd LL57 2UW, United Kingdom
Tobacco (Nicotiana plumbaginifolia) seedlings genetically transformed to
express apoaequorin were incubated in h-coelenterazine to reconstitute the
calcium-sensitive luminescent protein aequorin. Treatment of these
seedlings with hydrogen peroxide resulted in a transient burst of
calcium-dependent luminescence lasting several minutes. Even though the
hydrogen peroxide stimulus was persistent, the change in cytosolic free
calcium concentration ([Ca2+]cyt) was transient, suggesting the presence of
a refractory period. When seedlings were pretreated with hydrogen peroxide,
there was no increase in [Ca2+]cyt upon a second application, which
confirmed the refractory character of the response. Only when the two
treatments were separated by 4 to 8 hr was full responsiveness recovered.
However, treatment with hydrogen peroxide did not inhibit mobilization of
[Ca2+]cyt induced by either cold shock or touching, suggesting that these
three signals mobilize different pools of intracellular calcium. To examine
whether [Ca2+]cyt is regulated by the redox state of the cytoplasm, we
pretreated seedlings with buthionine sulfoximine (to modify cellular
glutathione levels) and inhibitors of ascorbate peroxidase. These
inhibitors modify the hydrogen peroxide-induced transients in [Ca2+]cyt,
which is consistent with their effects on the cellular
prooxidant/antioxidant ratio. Treatment with hydrogen peroxide that
elicited [Ca2+]cyt increases also brought about a reduction in superoxide
dismutase enzyme activity. This reduction could be reversed by treatment
with the calcium channel blocker lanthanum. This indicates that there is a
role for calcium in plant responses to oxidative stress.
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|
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|
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|
|
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|
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|
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|
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|
|
|
|
|
|
|
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|
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|
|
|
|
|
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|
|
|
|
|
|
|
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[Full Text]
[PDF]
|
|
|
|
|
|
|
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4097 - 4102.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Larkindale and M. R. Knight
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February 1, 2002;
128(2):
682 - 695.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Murata, Z.-M. Pei, I. C. Mori, and J. Schroeder
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PLANT CELL,
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13(11):
2513 - 2523.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Desikan, S. A.-H.-Mackerness, J. T. Hancock, and S. J. Neill
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September 1, 2001;
127(1):
159 - 172.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Zhang, L. Zhang, F. Dong, J. Gao, D. W. Galbraith, and C.-P. Song
Hydrogen Peroxide Is Involved in Abscisic Acid-Induced Stomatal Closure in Vicia faba
Plant Physiology,
August 1, 2001;
126(4):
1438 - 1448.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Jiang and B. Huang
Effects of calcium on antioxidant activities and water relations associated with heat tolerance in two cool-season grasses
J. Exp. Bot.,
February 1, 2001;
52(355):
341 - 349.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kawano, R. Pinontoan, N. Uozumi, C. Miyake, K. Asada, P. E. Kolattukudy, and S. Muto
Aromatic Monoamine-Induced Immediate Oxidative Burst Leading to an Increase in Cytosolic Ca2+ Concentration in Tobacco Suspension Culture
Plant Cell Physiol.,
November 1, 2000;
41(11):
1251 - 1258.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Blume, T. Nürnberger, N. Nass, and D. Scheel
Receptor-Mediated Increase in Cytoplasmic Free Calcium Required for Activation of Pathogen Defense in Parsley
PLANT CELL,
August 1, 2000;
12(8):
1425 - 1440.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Vanacker, T. L.W. Carver, and C. H. Foyer
Early H2O2 Accumulation in Mesophyll Cells Leads to Induction of Glutathione during the Hyper-Sensitive Response in the Barley-Powdery Mildew Interaction
Plant Physiology,
August 1, 2000;
123(4):
1289 - 1300.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
T. Kawano and S. Muto
Mechanism of peroxidase actions for salicylic acid-induced generation of active oxygen species and an increase in cytosolic calcium in tobacco cell suspension culture
J. Exp. Bot.,
April 1, 2000;
51(345):
685 - 693.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. D. McKersie, J. Murnaghan, K. S. Jones, and S. R. Bowley
Iron-Superoxide Dismutase Expression in Transgenic Alfalfa Increases Winter Survival without a Detectable Increase in Photosynthetic Oxidative Stress Tolerance
Plant Physiology,
April 1, 2000;
122(4):
1427 - 1438.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
G. Baum, J. C. Long, G. I. Jenkins, and A. J. Trewavas
Stimulation of the blue light phototropic receptor NPH1 causes a transient increase in cytosolic Ca2+
PNAS,
November 9, 1999;
96(23):
13554 - 13559.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Sanders, C. Brownlee, and J. F. Harper
Communicating with Calcium
PLANT CELL,
April 1, 1999;
11(4):
691 - 706.
[Full Text]
|
|
|
|
|
|
|
M. T. Marrè, E. Amicucci, L. Zingarelli, F. Albergoni, and E. Marrè
The Respiratory Burst and Electrolyte Leakage Induced by Sulfhydryl Blockers in Egeria densa Leaves Are Associated with H2O2 Production and Are Dependent on Ca2+ Influx
Plant Physiology,
December 1, 1998;
118(4):
1379 - 1387.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. Bouvier, R. A. Backhaus, and B. Camara
Induction and Control of Chromoplast-specific Carotenoid Genes by Oxidative Stress
J. Biol. Chem.,
November 13, 1998;
273(46):
30651 - 30659.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. G. Cessna, S. Chandra, and P. S. Low
Hypo-osmotic Shock of Tobacco Cells Stimulates Ca2+ Fluxes Deriving First from External and then Internal Ca2+ Stores
J. Biol. Chem.,
October 16, 1998;
273(42):
27286 - 27291.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. J. Turano and T. K. Fang
Characterization of Two Glutamate Decarboxylase cDNA Clones from Arabidopsis
Plant Physiology,
August 1, 1998;
117(4):
1411 - 1421.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Vanacker, T. L.W. Carver, and C. H. Foyer
Pathogen-Induced Changes in the Antioxidant Status of the Apoplast in Barley Leaves
Plant Physiology,
July 1, 1998;
117(3):
1103 - 1114.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Gong, A. H. van der Luit, M. R. Knight, and A. J. Trewavas
Heat-Shock-Induced Changes in Intracellular Ca2+ Level in Tobacco Seedlings in Relation to Thermotolerance
Plant Physiology,
January 1, 1998;
116(1):
429 - 437.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y Yang, J Shah, and D F Klessig
Signal perception and transduction in plant defense responses.
Genes & Dev.,
July 1, 1997;
11(13):
1621 - 1639.
[PDF]
|
|
|
|
|
|
|
R. Sanchez-Fernandez, M. Fricker, L. B. Corben, N. S. White, N. Sheard, C. J. Leaver, M. Van Montagu, D. Inze, and M. J. May
Cell proliferation and hair tip growth in the Arabidopsis root are under mechanistically different forms of redox control
PNAS,
March 18, 1997;
94(6):
2745 - 2750.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. S. Tiwari, B. Belenghi, and A. Levine
Oxidative Stress Increased Respiration and Generation of Reactive Oxygen Species, Resulting in ATP Depletion, Opening of Mitochondrial Permeability Transition, and Programmed Cell Death
Plant Physiology,
April 1, 2002;
128(4):
1271 - 1281.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
